Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of RJ423EV and RJ423c141 cells

ABSTRACT: RNA sequencing of RJ423 cells expressing an empty vector (RJ423EV) or a vector containing the miR-200c/141 cluster (RJ423c141)

ORGANISM(S): Mus musculus

PROVIDER: GSE150101 | GEO | 2021/02/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA sequencing of MDA-231EV, MDA-231c141 and MDA-231ba429 cells

Project description:RNA sequencing of MDA-MB-231 cells expressing an empty vector (MDA-231EV), a vector containing the miR-200c/141 cluster (MDA-231c141) or a vector containing the miR-200b/200a/429 cluster

2021-02-08 | GSE150099 | GEO

miRNA sequencing of RJ423EV and RJ423c141 cells

Project description:miRNA sequencing of RJ423 cells expressing an empty vector (RJ423EV) or a vector containing the miR-200c/141 cluster (RJ423c141)

2021-02-08 | GSE150106 | GEO

miRNA sequencing of MDA-231EV, MDA-231c141 and MDA-231ba429 cells

Project description:miRNA sequencing of MDA-M-231 cells expressing an empty vector (MDA231EV), a vector containing the miR-200c/141 cluster (MDA-231c141) or a vector containing the miR-200b/200a/429 cluster (MDA-231ba429)

2021-02-08 | GSE150104 | GEO

MDA-231EV, MDA-231c141 and MDA-231ba429 cells following injection of the cells into mammary glands of NCG and subsequent mammary tumor development

2022-04-11 | GSE193479 | GEO

miRNA sequencing of RJ423EV and RJ423ba429 cells

Project description:miRNA sequencing of RJ423 cells expressing an empty vector (RJ423EV) or a vector containing the miR-200b/200a/429 cluster (RJ423ba429)

2021-02-08 | GSE150105 | GEO

RNA sequencing of RipmiR-141~200c and RipmiR-141~200c Zeb1200M islets

Project description:We performed RNA sequencing of islets of Langerhans isolated from RipmiR-141~200c and RipmiR-141~200c Zeb1200M mice to determine the transcriptomic effects of mutating miR-200 binding sites in the endogenous Zeb1 3'UTR of mice in which miR-141~200c is overexpressed under the rat insulin promoter (RIP).

2022-04-30 | E-MTAB-10537 | biostudies-arrayexpress

Global changes in gene expression in response to miR-200 overexpression in 4TO7 cells

2010-12-23 | GSE19631 | GEO

Global changes in gene expression in response to miR-200 overexpression in 4TO7 cells

Project description:Transcriptional profiling of 4TO7 cells stably overexpressing miR-200s or CDH1. We observe a dramatic shift in the gene signatures when miR-200s (cluster 2; miR-200c/141, or both clusters) are overexpressed relative to controls. However, cluster 1 overexpression (miRs-200b/a/429) alone or CDH1 overexpression does not induce global changes in gene expression to levels observed for cluster 2 (miR-200c/141) or both clusters together. One cell line (4TO7) cells: four different overexpression experiments, Cluster 1, Cluster 2, Clusters 1+2, CDH1, and respective controls, Puro vec, Hygro vec, Puro+Hygro vec and Puro vec. Each experiment has two biological replicates. Total, 16 samples.

2010-12-23 | E-GEOD-19631 | biostudies-arrayexpress

MicroRNA-200c/141 confines ERα activity and expression pattern by targeting Ccnd1 [bulk RNA-seq]

Project description:ERα is one of the most important transcription factors and therapeutic targets in breast cancer. The patterns of ERα expression in normal breast tissue and cancerous lesions are strikingly different. What drives the change of ERα pattern during lesions formation remains unclear. Here, we describe a novel regulatory mechanism through which miR-200c/141 regulates the level as well as the distribution of ERα in the mammary gland. miR-200c/141 is specifically expressed in luminal cells. Luminal-deletion of miR-200c/141 (miR-cKO) leads to a drastic expansion of ERα+ cell proportion. Single cell RNAseq reveals that miR-cKO generates aberrant luminal subpopulations with increased proliferative and anti-apoptotic features. In vivo lineage tracing of ER- luminal cells demonstrates that miR-200c/141 deletion can convert ER- cells into ER+ cells, which contribute partly to the increase of ERα+ luminal cells. Mechanistic study identifies Ccnd1 as a novel target of miR-200c/141 . Upon miR-200c/141deletion, elevated Ccnd1 level corroborates ERα transcriptional activation, leads to enhanced ERα signaling activity, consequently increased transcription of ERα coding gene Esr1 through self-activation at promoter E1. Our findings reveal a new miR-200c/141-Ccnd1-ERα axis, and provide new molecular insights into how miR-200c/141-Ccnd1 enforces the lineage barrier between ER- and ER+ luminal cells, and what drives the change of ERα expression pattern.

2021-05-05 | GSE173815 | GEO

MicroRNA-200c/141 confines ERα activity and expression pattern by targeting Ccnd1 [single-cell RNA-seq]

2021-05-05 | GSE173816 | GEO