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Single cell profiling of hemogenic endothelium and its surrounding niche in the mouse dorsal aorta

ABSTRACT: The first hematopoietic stem cells originate from hemogenic endothelium (HE), that trans-differentiate into the lumen to form hematopoietic clusters. The molecular mechanisms driving this transition are only poorly understood. Here, we performed single cell RNA-seq profiling of HE cells utilising a RUNX1 and GFI1 reporter mouse line.This allowed for a detailed characterisation of HE cells during endothelial-to-hematopoietic transition. Additionally we profiled the cells surrounding the dorsal aorta. These dataset present a comprehensive profile at single cell resolution of cells invovled in the endothelial-to-hematopoietic transition and the surrounding dorsal aorta niche.

ORGANISM(S): Mus musculus

PROVIDER: GSE150412 | GEO | 2021/09/21

REPOSITORIES: GEO

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