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Molecular analysis of the subaortic hematopoietic stem cell niche [E11.5]

ABSTRACT: Hematopoietic stem cells (HSCs) are formed in the Aorta-Gonad-Mesobephros (AGM) region of mouse midgestation embryos. This process is tightly regulated in time and space by surrounding cells inculding mesenchymal cells located underneath the dorsal aorta. We combined laser microdissection and microarrays to isolate the dorsal and ventral aortic tissues at three developmental stages and to explore their gene expression profiling.

ORGANISM(S): Mus musculus

PROVIDER: GSE159590 | GEO | 2024/03/11

REPOSITORIES: GEO

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Molecular analysis of the subaortic hematopoietic stem cell niche [E12.5]

Project description:Hematopoietic stem cells (HSCs) are formed in the Aorta-Gonad-Mesobephros (AGM) region of mouse midgestation embryos. This process is tightly regulated in time and space by surrounding cells inculding mesenchymal cells located underneath the dorsal aorta. We combined laser microdissection and microarrays to isolate the dorsal and ventral aortic tissues at three developmental stages and to explore their gene expression profiling.

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