Analysis of differential gene expression in a Streptococcus sanguinis ssaACB deletion mutant before and after addition of EDTA to deplete cellular manganese

ABSTRACT: The purpose of this study was to determine why manganese is required for the growth of Streptococcus sanguinis. The study employed a mutant derivative of S. sanguinis strain SK36 deleted for the genes encoding the manganese transporter SsaACB. The mutant strain, JFP169, was grown in a fermenter with constant aeration in BHI broth. RNA-seq was used to compare gene expression from samples taken 20 minutes prior to addition of 100 micromolar EDTA to samples taken 10, 25, and 50 min after addition. Samples were treated with xx to deplete ribosomal RNA, then sequenced on a MiSeq sequencer, producing paired-end 150-base reads. Sequences were trimmed and filtered and expression levels compared using the DE-Seq module in the Geneious Prime program.

ORGANISM(S): Streptococcus sanguinis

PROVIDER: GSE150593 | GEO | 2020/10/17

REPOSITORIES: GEO