Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genetic screening for single-cell variability modulators driving therapy resistance [WM989-A6-G3-Cas9 5a3]

ABSTRACT: We present the transcriptome of WM989-A6-G3-Cas9 5a3 melanoma cells followng the knockout (via CRISPR-Cas9) of different proteins we identified as modulators the frequency of cellular states associated with resistance to mutant-BRAF inhibition therapy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE151825 | GEO | 2020/06/05

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genetic screening for single-cell variability modulators driving therapy resistance [WM989-A6-G3-Cas9 5a3]

Project description:Genetic screening for single-cell variability modulators driving therapy resistance [WM989-A6-G3-Cas9 5a3]

| PRJNA637377 | ENA

Agrobacterium tumefaciens A6 strain:A6

Project description:Agrobacterium tumefaciens A6 strain:A6 Genome sequencing

| PRJNA236422 | ENA

Stenotrophomonas sp. a6 strain:a6

Project description:Stenotrophomonas sp. a6 strain:a6 Genome sequencing

| PRJNA1003146 | ENA

Scenedesmus sp. A6 strain:A6

Project description:Scenedesmus sp. A6 strain:A6 Genome sequencing

| PRJNA475403 | ENA

clampFISH 2.0 enables rapid, scalable amplified RNA detection in situ

Project description:We developed an amplified single-molecule RNA FISH method called clampFISH 2.0. To validate its performance, we used clampFISH 2.0 on two cell lines, WM989 A6-G3 cells and WM989 A6-G3 cells grown in vemurafenib, and across 10 genes, compared clampFISH 2.0 spot counts to the FPKM values from bulk RNA sequencing.

2022-08-23 | GSE211491 | GEO

Regulation of somatosensory mechanotransduction by Annexin A6

Project description:Sensory neuron mechanically-activated slowly adapting currents have been linked to noxious mechanosensation. We identified a Conotoxin, Noxious Mechanosensation Blocker -1, that blocks such currents selectively. Using an active biotinylated form of the toxin we identified 67 binding proteins in sensory neurons and sensory neuron-derived cell lines using mass spectrometry. Annexin A6 was the most frequently identified binding protein. Annexin A6 knockout mice showed an enhanced sensitivity to mechanical stimuli. Rapidly adapting currents were enhanced and slowly adapting channels diminished. The percentage of neurons expressing slowly adapting currents fell and non-responsive neurons increased. Conversely, overexpression of annexin A6 in DRG neurons inhibited rapidly adapting currents without effects on slowly adapting currents. Co-expression of annexin A6 with Piezo 2 led to an inhibition of Piezo-mediated rapidly adapting currents. AAV-mediated gene delivery of annexin A6 to sensory neurons in a mouse model of osteoarthritis attenuated mechanical pain. These data demonstrate a role for annexin A6 in somatosensory mechanotransduction.

2018-04-17 | PXD005718 | Pride

Trichomonas vaginalis G3 strain:G3

Project description:Trichomonas vaginalis G3 strain:G3 Raw sequence reads

| PRJNA290805 | ENA

Lecanicillium sp. G3 strain:G3

Project description:Lecanicillium sp. G3 strain:G3 Genome sequencing and assembly

| PRJNA1073173 | ENA

Vibrio parahaemolyticus strain:A6 | isolate:A6

Project description:Vibrio parahaemolyticus strain:A6 | isolate:A6 Genome sequencing and assembly

| PRJNA913556 | ENA

Genetic screening for single-cell variability modulators driving therapy resistance

Project description:We present the transcriptome of WM989 melanona cells without or without inhibition of DOT1L. Different samples represent either the bulk population of melanoma cells, or subpopulations that express high or low levels of the drug resistance markers EGFR and NGFR

2020-04-25 | GSE149280 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data