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Characterizing gene expression changes within the human hair follicle bulb driven by hepatocyte growth factor secreted by perifollicular fat

ABSTRACT: Dermal white adipose tissue (dWAT) has been linked to modulation of murine hair follicle growth and cycling, however no studies have characterized its effects upon human hair growth. Since HGF was detected as a dWAT secretion product in HF-dWAT co-culture, the aim of this study was to characterize changes in gene expression in human dissected hair follicles vs. follicles cultured with surrounding dermal fat treated with/without a neutralizing antibody to HGF. To this end, the hair matrix and pre-cortex in each condition was isolated using laser-capture microdissection and microarray was carried out on the amplified cDNA. We identified 593 genes differentially modulated owing to the dWAT-derived HGF i.e. 301 up-regulated genes and 292 down-regulated genes in the presence of dWAT-secreted HGF and vice versa when HGF is neutralized.

ORGANISM(S): Homo sapiens

PROVIDER: GSE151972 | GEO | 2023/05/29

REPOSITORIES: GEO

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