Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of mouse after single sevoflurane exposure

ABSTRACT: we combined RNA sequencing analysis of mouse prefrontal cortex after single sevoflurane exposure

ORGANISM(S): Mus musculus

PROVIDER: GSE152149 | GEO | 2023/06/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

10x single cell sequencing for primary neurons transcriptomes after sevoflurane exposure

Project description:We thus isolated cortex from mice, with or without sevoflurane exposure, and performed single cell sequencing. Single cell sequencing is a innovative experimental procedures which allows the identification of various subsets of cells in the neverous system after sevoflurane exposure. Isolated cells were concentrated to 1000 cells/uL and approximately 17,000 cells were loaded into each channel to generate single-cell Gel Bead-In-Emulsions (GEMs), which led to expected mRNA barcoding of 10,000 single-cells for each sample. Seurat package was used for cell normalization and regression. Sevoflurane and control groups were aligned and combined using Seurat. Using t-distributed stochastic neighbor embedding (tSNE), 13 cluters of cells were visualized. Cells were assigned to various clusters based on strong expression of specific genes.

2022-07-13 | GSE139012 | GEO

RNA sequencing of Macaca mulatta prefrontal cortex after sevoflurane exposure

Project description:RNA sequencing of Macaca mulatta prefrontal cortex after sevoflurane exposure

| PRJNA624269 | ENA

RNA sequencing of mouse after single sevoflurane exposure

Project description:RNA sequencing of mouse after single sevoflurane exposure

| PRJNA638413 | ENA

sevoflurane exposures on neonatal and adult mice,LC-MSMS

Project description:Sevoflurane is the most commonly used general anesthetic in pediatric surgery, but it has the potential to be neurotoxic. Previous research found that long-term or multiple sevoflurane exposures could cause cognitive deficits in newborn mice but not adult mice, whereas short-term or single inhalations had little effect on cognitive function at both ages. The mechanisms behind these effects, however, are unclear. In the current study, 6- and 60-day-old C57bl mice in the sevoflurane groups were given 3% sevoflurane plus 60% oxygen for three consecutive days, each lasting 2 hours, while those in the control group only got 60% oxygen. The cortex tissues were harvested on the 8th or 62nd day. The tandem mass tags (TMT)pro-based quantitative proteomics combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysi were applied to analyze the influences of multiple sevoflurane anesthesia on the cerebral cortex in mice with various ages. A total of 6247 proteins were measured using the combined quantitative proteomics methods of TMTpro-labeled and LC-MS/MS, 443 of which were associated to the age-dependent neurotoxic mechanism of repeated sevoflurane anesthesia. Our findings would help to further the mechanistic study of age-dependent anesthetic neurotoxicity and contribute to seek for effective protection in the developing brain under general anesthesia.

2022-12-08 | PXD037294 | Pride

RNA sequencing of rhesus macaque prefrontal cortex and human embryonic stem cell after sevoflurane exposure

Project description:RNA sequencing of rhesus macaque prefrontal cortex and human embryonic stem cell after sevoflurane exposure

| PRJNA624266 | ENA

m6A-Seq analysis for the methylated RNA of primate prefrontal cortex after sevoflurane exposure

Project description:m6A-Seq analysis for the methylated RNA of primate prefrontal cortex after sevoflurane exposure

| PRJNA731398 | ENA

RNA sequencing of Macaca mulatta prefrontal cortex after sevoflurane exposure

Project description:RNA sequencing analysis of macaque’s prefrontal cortex

2020-09-10 | GSE148437 | GEO

Sevoflurane Exposure Alters Long Non-coding RNA and Messenger RNA Profiles in the Neonatal Mouse Hippocampus

Project description:The focus of current study is to investigate the effect of developing sevoflurane exposure on lncRNA profiles and molecular signaling involvement in neurotoxicity. An Expression Microarray assay (V3.0) and data analysis services were provided by Arraystar Inc. (Rockville, MD) to assess and compare the global abundance of 27,427 lncRNAs and 18,855 mRNAs in the hippocampi of mice exposed to either sevoflurane or normal air controls.

2021-02-17 | GSE155770 | GEO

10x single cell sequencing for primary neurons transcriptomes after sevoflurane exposure

Project description:10x single cell sequencing for primary neurons transcriptomes after sevoflurane exposure

| PRJNA578089 | ENA

RNA sequencing of human embryonic stem cell after sevoflurane exposure

Project description:RNA sequencing of human embryonic stem cell after sevoflurane exposure

| PRJNA624268 | ENA