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Biocompatibility and Discovery of the Potential Applications of Magnetite (Fe3O4) Nanoparticles

ABSTRACT: A Transcriptomics Approach to Study the Biocompatibility and Finding out the Potential Applications of Magnetite (Fe3O4) Nanoparticles Here in this study, we examine the molecular effects of uptake of Fe3O4 nanoparticles using a whole genome microarray study in human epithelial cancer cell line. 38 genes (55%) out of 69 downregulated genes were found to be associated with TGF-beta or BMP signaling including six genes, Id1, Id2, Id3, Caspase-9, Smad6 and SMAD7, important negative regulators of these signaling pathways involved in development and tumorigenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE15248 | GEO | 2010/03/13

SECONDARY ACCESSION(S): PRJNA116539

REPOSITORIES: GEO

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Homo sapiens

Project description:Biocompatibility and Discovery of the Potential Applications of Magnetite (Fe3O4) Nanoparticles

| PRJNA116539 | ENA

Genome binding/occuoancy profiling by high throughout sequencing in Wild Type and ID1/3 double knockout hESCs

Project description:TCF3 belongs to E protein family and has the function of transcription factor. TCF3 can directly interact with HLH family proteins ID1 and ID3 to play biological functions. ID1 and ID3 do not have the ability to directly bind to DNA and need to be regulated by TCF3. We found changes in pluripotency in ID1 and ID3 double knockout lines of human ESC, so we suspected that ID1 / 3 played a regulatory role through TCF3. Therefore, TCF3 ChIP-seq was done in WT and KO lines, hoping to find the downstream genes regulated by ID1 / 3 through TCF3, and then regulate hESC pluripotency through these genes.

2022-06-27 | GSE183518 | GEO

The Id-RAP1 axis is essential for glioma maintenance and residency of glioma cells in the perivascular niche

Project description:The experiment was designed to achieve Cre recombinase mediated deletion of Id1, Id2 and Id3 in a temporally controlled fashion in tumor cells of Id1, Id2, Id3 floxed mice with the aim of comparing the gene expression profiles of Id expressing versus Id deleted tumors.

2013-04-30 | E-MTAB-1303 | biostudies-arrayexpress

In vitro biocompatibility of ZrO2 vs Y-TZP alloys on Saos-2 cells: influence of composition and surface topography

Project description:This study assess the biocompatibility of ZrO2 vs Y-TZP alloys on Saos-2 cells, through the combination of conventional toxicological assay, morphological observations and transcriptomic analysis performed on Saos-2 cells. The biocompatibility of ZrO2 alloys was evaluated in comparison with Y-TZP alloys, studying cell proliferation (WST-1) and cell morphology/adhesion (SEM) on human Saos-2 cell lines. Finally, an evaluation of gene expression was performed on Y-TZP alloys rough surface that seems more interesting regarding biocompatibility results.

2022-07-13 | GSE203572 | GEO

In vitro biocompatibility of Ti-Cp vs Ti-26Nb alloys on Saos-2 and THP-1 cells

Project description:This study assess the biocompatibility of Ti-26Nb compared to Ti-Cp, a reference in implantology, through the combination of conventional toxicological assay, morphological observations and transcriptomic analysis performed on two different cells line : Saos-2 and THP-1. The biocompatibility of Ti-Cp implants was evaluated in comparison with Ti-26Nb implants both having a mirror polished surface, studying cell proliferation (trypan blue and WST-1) and cell morphology/adhesion (SEM) on human THP-1 and Saos-2 cell lines. Finally, an evaluation of a potential toxicity of potassium niobiate powder KNbO3 was carried out by measuring metabolic activity and realizing a transcriptomic study on the THP-1 line (the first one) to deepen the results observed with Ti-26Nb discs. Indeed, niobiate ions are potentially released from implant in cellular acidic environnement. Concerning Saos-2 cells, our results suggest that Ti-26Nb and Ti-Cp discs do not impact proliferation and viability. Concerning THP-1 cell line, a decrease in proliferation and viability is observed in contact of Ti-26Nb discs (hypothesis of a cell-line effect ?, no biocompatibility study on monocyte/macrophage cell lines such as THP-1 actually available in literature). KNbO3 powder does not impact viability of THP-1 cells and does not induce changes at molecular level. Taken together, these data suggest a possible toxicity of Ti-26Nb toward THP-1 cells not directly related to the niobium but perhaps to the manufacturing process of Titane niobium alloy.

2022-08-31 | GSE197987 | GEO

Id1 and Id3 Maintain Steady State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration

Project description:Single cell RNA-Seq was performed on ID1 ID3 null endothelial cells to determine the effects of ID gene loss on endothelial cell maintainence and growth.

2020-04-22 | GSE149030 | GEO

Improved Biocompatibility of Amino-functionalized Graphene Oxide in Caenorhabditis elegans

Project description:Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. Here, we study the adverse effects of GO and amino-functionalized GO (GONH2) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Seq data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. Our work establishes the enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as biomedical nanomaterial.

2022-05-10 | GSE131070 | GEO

Transcriptomic profiling of 4T1 cells depleted for Id1/3 and Robo1

Project description:Id1 and its closely related family member Id3 are expressed by a diversity of stem and progenitor cells. We show that Id1/3 are required for the self-renewal and proliferation of triple negative breast cancer (TNBC) cells both in vitro and in vivo. Furthermore, we identified that Id1/3 negatively regulates the tumour suppressor gene Robo1. Depletion of Robo1 could rescue the proliferative defect induced by Id1/3 knockdown. To understand the mechanisms by which Robo1 rescues cell proliferation in Id1/3 depleted cells, we performed RNA-Sequencing on 4T1 cells with Dox-inducible Id1/3 KD and/or Robo1 depletion using siRNA. We conclude that following Id1/3 knockdown, Robo1 is induced and exerts anti-proliferative effects via suppression of a Myc transcriptional program.

2020-10-31 | GSE129858 | GEO

Three human cell types respond to multi-walled carbon nanotubes and titanium dioxide nanobelts with cell-specific transcriptomic and proteomic expression [SAE cells]

Project description:To identify key biological pathways that define toxicity or biocompatibility after nanoparticle exposure, three human cell types were exposed in vitro to two high aspect ratio nanoparticles for 1 hr or 24 hr and collected for global transcriptomics. Transcriptional responses were measured by global microarray analysis of cells in culture. Groups (N=3 biological replicates) of SAE cells exposed to 0, 10 or 100 ug/ml MWCNT or TiO2-NB nanoparticles for 1 or 24 hr.

2012-12-15 | E-GEOD-42067 | biostudies-arrayexpress

Three human cell types respond to multi-walled carbon nanotubes and titanium dioxide nanobelts with cell-specific transcriptomic and proteomic expression [Caco-2/HT29-MTX cells]

Project description:To identify key biological pathways that define toxicity or biocompatibility after nanoparticle exposure, three human cell types were exposed in vitro to two high aspect ratio nanoparticles for 1 hr or 24 hr and collected for global transcriptomics. Transcriptional responses were measured by global microarray analysis of cells in culture. Groups (N=3 biological replicates) of Caco-2/HT29-MTX cells exposed to 0, 10 or 100 ug/ml MWCNT or TiO2-NB nanoparticles for 1 or 24 hr.

2012-12-15 | E-GEOD-42066 | biostudies-arrayexpress

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