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Transcriptomic analysis of APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells.

ABSTRACT: We performed RNA sequencing analysis of two human colon epithelial cell lines (HCEC 1CT_WT and HCEC 1CT_APC KD).

ORGANISM(S): Homo sapiens

PROVIDER: GSE152562 | GEO | 2020/09/25

REPOSITORIES: GEO

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Transcriptomic analysis of APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells.

Project description:In order to investigate sophisticated events during cancer initiation, we established early colorectal cancer development model. We performed scRNA-seq and analyzed the scRNA-seq dataset. scRNA-seq was conducted at 3- and 7-days after transduction of shRNA targeting APC (shAPC) and scrambled shRNA (shScr) on HCEC 1CT cells.

2020-09-25 | GSE152745 | GEO

APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells

Project description:APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells

| PRJNA640310 | ENA

Transcriptomic analysis of APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells.

Project description:Transcriptomic analysis of APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells.

| PRJNA640318 | ENA

Transcriptomic analysis of APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells.

Project description:Transcriptomic analysis of APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells.

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APC knock down HCEC 1CT cells compared to the wild type HCEC 1CT cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-09-25 | GSE152746 | GEO

Human Corneal Epithelial Cells (SV40-HCEC) 6h Treatment with 100nM Vitamin D (1,25D3) vs. Vehicle Control

Project description:Inflammatory signals must be regulated and kept in check in order to prevent tissue damage.Â This is especially true in the cornea, where damage can induce loss of transparency, essential for vision.Â Toll-like receptors (TLRs) are present at the ocular surface and, in addition to being protective against infection, have also been implicated in the pathogenesis of dry eye syndrome, an inflammatory condition that affects millions of individuals in the United States each year.Â Â Therefore, an important area of research is the development of new anti-inflammatory therapeutics that limit aberrant ocular surface inflammation.Â Vitamin D has been studied for its role in suppressing inflammation in other tissues.Â In previous studies, we have demonstrated that vitamin D is able to decrease proinflammatory mediators induced by TLRs in human corneal epithelial cells (HCEC).Â Therefore, the goal of the current study was to examine this mechanism further through an evaluation of vitamin Dâ??s influence on gene expression in two different HCEC cell lines. Microarray comparing SV40-HCEC, human corneal epithelial cells, treated for 6 hours with Vitamin D (1,25D3) and control (0.1% ethanol) Two-condition arrays: SV40-HCEC cells were treated with either 100nM 1,25D3 or 0.1% Ethanol for 6h. Two biological replicates, one per array, with a dye swap (technical replicate).

2016-09-01 | E-GEOD-72663 | biostudies-arrayexpress

Human Corneal Epithelial Cells (SV40-HCEC) 6h Treatment with 100nM Vitamin D (1,25D3) vs. Vehicle Control

Project description:Inflammatory signals must be regulated and kept in check in order to prevent tissue damage. This is especially true in the cornea, where damage can induce loss of transparency, essential for vision. Toll-like receptors (TLRs) are present at the ocular surface and, in addition to being protective against infection, have also been implicated in the pathogenesis of dry eye syndrome, an inflammatory condition that affects millions of individuals in the United States each year. Therefore, an important area of research is the development of new anti-inflammatory therapeutics that limit aberrant ocular surface inflammation. Vitamin D has been studied for its role in suppressing inflammation in other tissues. In previous studies, we have demonstrated that vitamin D is able to decrease proinflammatory mediators induced by TLRs in human corneal epithelial cells (HCEC). Therefore, the goal of the current study was to examine this mechanism further through an evaluation of vitamin D’s influence on gene expression in two different HCEC cell lines. Microarray comparing SV40-HCEC, human corneal epithelial cells, treated for 6 hours with Vitamin D (1,25D3) and control (0.1% ethanol)

2016-09-01 | GSE72663 | GEO

Rapid conditional knock-down-knock-in system for mammalian cells.

Project description:RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound phenotypes, as many proteins combine multiple functions within one molecule and coordinate the assembly of multimolecular complexes. Replacing the endogenous protein with ectopic wild-type or mutant forms can exclude off-target effects, preserve complexes and unravel specific roles of domains or modifications. Therefore, we developed a rapid-knock-down-knock-in system for mammalian cells. Stable polyclonal cell lines were generated within 2 weeks by simultaneous selection of two episomal vectors. Together these vectors mediated reconstitution and knock-down in a doxycycline-dependent manner to allow the analysis of essential genes. Depletion was achieved by an artificial miRNA-embedded siRNA targeting the untranslated region of the endogenous, but not the ectopic mRNA. To prove effectiveness, we tested 17 mutants of WDR12, a factor essential for ribosome biogenesis and cell proliferation. Loss-off function phenotypes were rescued by the wild-type and six mutant forms, but not by the remaining mutants. Thus, our system is suitable to exclude off-target effects and to functionally analyze mutants in cells depleted for the endogenous protein.

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TYRP1 knock-down in human melanoma cell line (501Mel)

Project description:Efficient TYRP1 knock-down (KD) have been performed in human melanoma cell line expressing a high level of TYRP1 mRNA and protein in order to identify the molecular pathway explaining the decrease of cell proliferation in TYRP1 KD cells.

2017-07-01 | GSE70561 | GEO

USP7 shRNA knock-down in T-ALL cells [Jurkat_HSB2_shRNA_RNA-seq]

Project description:RNAseq analysis of USP7 shRNA KD in T-ALL cell lines. USP7 shRNAs lentivirus was generated by co-transfecting 293T cells with shRNA vectors (OriGene or Sigma). T-ALL cells were transduced with USP7 shRNA lentivirus and sorted for GFP positive cells five days after transduction or selected by puromycin.

2021-03-09 | GSE148522 | GEO

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