Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TYRP1 knock-down in human melanoma cell line (501Mel)

ABSTRACT: Efficient TYRP1 knock-down (KD) have been performed in human melanoma cell line expressing a high level of TYRP1 mRNA and protein in order to identify the molecular pathway explaining the decrease of cell proliferation in TYRP1 KD cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE70561 | GEO | 2017/07/01

SECONDARY ACCESSION(S): PRJNA289013

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

NFATC2 knock-down on melanoma cell line A375

Project description:RNA-seq on NFATC2 knock-down (siRNA) on melanoma cell line A375

2017-09-29 | GSE99466 | GEO

A noncoding function of TYRP1 mRNA promotes melanoma growth

Project description:Competition among RNAs to bind miRNA is proposed to influence biological systems. However, the role of this competition in disease onset is unclear. Here, we report that TYRP1 mRNA, in addition to encoding tyrosinase-related protein 1 (TYRP1), indirectly governs cell proliferation by sequestering miR-16 to non-canonical miRNA response elements (MREs). Consequently, the sequestered miR-16 is no longer able to repress its mRNA targets, such as RAB17, which is involved in melanoma cell proliferation and tumor growth. Restoration of miR-16 tumor suppressor function can be achieved in vitro by silencing TYRP1 or increasing miR-16 expression. Importantly, TYRP1-dependent miR-16 sequestration can also be overcome in vivo by using small oligonucleotides that mask miR-16 binding sites on the TYRP1 mRNA. Together, our findings assign a pathogenic noncoding function to the TYRP1 mRNA and highlight miRNA displacement as a new targeted therapeutic approach in melanoma.

2017-08-08 | GSE95589 | GEO

CRISPR/Cas9 knock down line

Project description:CRISPR/Cas9 knock down line

| PRJNA521684 | ENA

RNA Sequencing of melanoma cell line (WM983B) upon Fbxo4 knock-down

Project description:Fbxo4 is a member of Skip1-Cullin1-F-box protein E3 ubiquitin ligase complex that controls RNA biding proteins. We report that loss of Fbxo4 in melanoma contribute to altered gene expression leading to dysregulation pathways reponsible for cell-cell interaction, cell anocharege, invasion and motility.

2022-09-21 | GSE196396 | GEO

FGFR3 knock-down

Project description:FGFR3 knock-down

| PRJNA330963 | ENA

SPARC knock down

Project description:SPARC knock down

| PRJNA327124 | ENA

Transcriptomic analysis of ATP6V1A knock-down and amyloid beta-treated neurons

Project description:ATP6V1A plays a unique role in synapse function in neurons and we found decreased neuronal activity in ATP6V1A-deficient neurons. To characterize the molecular pathways regulated by ATP6V1A under both normal and stressed conditions, we generated hiPSC-derived NGN2-neurons with reduced ATP6V1A expression by CRISPRi knock-down (KD) and performed RNA-seq analysis on the wild-type and KD neurons which were subject to amyloid-beta or vehicle treatment. A number of gene ontology (GO)/pathways were identified in KD neurons without amyloid-beta treatment (proton transporting V-ATPase complex, phagosome acidification and trivalent inorganic cation transport were down-regulated, and mitochondrial protein complex up-regulated), while KD in amyloid-beta treated cells specifically resulted in down-regulation of cell adhesion, synapse assembly and structure/activity and up-regulation of UPR and ER stress response.

2020-10-30 | GSE128367 | GEO

Transcriptomic profiling of CDCP1 knock-down in human cardiac fibroblasts (HCFs)

Project description:To investigate the function of CDCP1 in regulation of HCF proliferation, we cultured primary HCFs and transfected with non-targeting siRNA and CDCP1 siRNAs. Total RNA samples were extracted for RNA-seq to identify transcriptome-wide gene expression changes in the HCFs after CDCP1 knock-down (KD).

2023-09-22 | GSE222695 | GEO

Expression data from knock-down ES stable cell line

Project description:In order to identify the effects of the knock-down of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the knock-down cell line. Transcriptome analysis of the knock-down transgenic mouse ES cell line. The knock-down cell line (shE13) was generated by stably expressing a specific short-hairpin RNA against E13 sequence thus knocking-down E13 expression in parental mouse ES cell line E14Tg2a.4 (E14, Hooper M et al., 1987). The specific mouse gene knocked down in the ES cell line is E130012A19Rik.

2012-12-13 | GSE31165 | GEO

RNA sequencing of PD-L1 knock down in uveal melanoma cells

Project description:In order to study the mechanism of nuclear PD-L1 in uveal melanoma, we knocked down PD-L1 and applied RNA-seq and CUT&Tag to identify its target genes in uveal melanoma.

2023-04-04 | GSE202884 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data