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Characterizing RNA stability genome-wide through combined analysis of PRO-seq and RNA-seq data

ABSTRACT: The rate at which RNA molecules decay is a key determinant of cellular RNA concentrations, yet current approaches for measuring RNA half-lives are generally labor-intensive, limited in sensitivity, and/or disruptive to normal cellular processes. Here we introduce a simple method for estimating relative RNA half-lives that is based on two standard and widely available high-throughput assays: Precision Run-On and sequencing (PRO-seq) and RNA sequencing (RNA-seq). Our method treats PRO-seq as a measure of transcription rate and RNA-seq as a measure of RNA concentration, and estimates the rate of RNA decay required for a steady-state equilibrium.

ORGANISM(S): Homo sapiens

PROVIDER: GSE153200 | GEO | 2020/12/15

REPOSITORIES: GEO

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