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Integrative profiling of early host chromatin accessibility responses in human neutrophils with sensitive pathogen detection [ATAC-seq]

ABSTRACT: ATAC-seq and RNA-seq of primary human neutrophils challenged with multiple pathogen mimicking ligands and whole organisms.

ORGANISM(S): Homo sapiens

PROVIDER: GSE153520 | GEO | 2020/06/30

REPOSITORIES: GEO

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Integrative profiling of early host chromatin accessibility responses in human neutrophils with sensitive pathogen detection [RNA-seq]

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Project description:We have performed analyses of murine primary bone marrow derived neutrophils challenged with either ultra-low dose or high dose of LPS. Neutrophils can be differentially programmed to distinct states by varying dosages of LPS. Purified bone marrow neutrophils were treated with PBS, 100 pg/ml LPS or 100 ng/ml LPS overnight, and harvested for scRNAseq analysis to examine their profiles of gene expression.

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