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Transcriptomic analysis of hepatotoxicity induced by pesticides imazalil, thiacloprid and clothianidin alone or in binary mixtures in a 28-day study in female Wistar rats

ABSTRACT: Co-occurrence of pesticide residues in food commodities raises a safety issue as their mixture effects on human health is largely unknown and need to be investigated. In a previous study, we reported the toxicological effects (pathology & histopathology) of imazalil (IMZ), thiacloprid (THI), and clothianidin (CTD) alone and in binary mixtures in a 28-day gavage study in female Wistar rats. Here, we undertook a transcriptomic analysis by means of total RNA sequencing (RNA-Seq). Ingenuity pathway analysis (IPA) was used to gain mechanistic information on the hepatotoxic pathways affected after treatment with pesticides, alone and in mixtures. Our data shows that 2986 genes were deregulated by CTD while IMZ and THI had effect on 194 and 225 genes, respectively. All three compounds share a common subset of genes whose network is associated with xenobiotic metabolism and nuclear receptors. Additionally, DNA repair process was predicted for CTD. The same networks were retrieved for the mixtures. Dose addition was indicated for the evaluated gene expressions. Our results bring new insight in the hepatotoxicity mechanisms of IMZ, THI, and CTD.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE153986 | GEO | 2020/12/31

REPOSITORIES: GEO

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Transcriptomic analysis of hepatotoxicity induced by pesticides imazalil, thiacloprid and clothianidin alone or in binary mixtures in a 28-day study in female Wistar rats

Project description:Transcriptomic analysis of hepatotoxicity induced by pesticides imazalil, thiacloprid and clothianidin alone or in binary mixtures in a 28-day study in female Wistar rats

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Stimulative effect of low-dose neonicotinoid pesticide, clothianidin on human neuroblastoma SH-SY5Y cells by disrupting intracellular signaling

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2019-02-06 | GSE126103 | GEO

Transcriptomics analysis of hepatotoxicity induced by the pesticides imazalil, thiacloprid and clothianidin alone or in binary mixtures in a 28-day study in female Wistar rats.

Project description:Co-occurrence of pesticide residues in food commodities raises a potential safety issue as their mixture effects on human health are largely unknown. In a previous study, we reported the toxicological effects (pathology and histopathology) of imazalil (IMZ), thiacloprid (THI), and clothianidin (CTD) alone and in binary mixtures in a 28-day oral gavage study in female Wistar rats. Five dose levels (up to 350 mg/kg body weight/day) ranging from a typical toxicological reference value to a clear effect dose were applied. In the present study, we undertook a transcriptomics analysis of rat livers by means of total RNA sequencing (RNA-Seq). Bioinformatic data analysis involving Ingenuity Pathway Analysis (IPA) was used to gain mechanistic information on hepatotoxicity-related pathways affected after treatment with the pesticides, alone and in mixtures. Our data show that 2986 genes were differentially regulated by CTD while IMZ and THI had effects on 194 and 225 genes, respectively. All three individual compounds shared a common subset of genes whose network is associated with xenobiotic metabolism and nuclear receptor activation. Similar networks were retrieved for the mixtures. Alterations in the expression of individual genes were in line with the assumption of dose addition. Our results bring new insight into the hepatotoxicity mechanisms of IMZ, THI, and CTD and their mixtures.

| S-EPMC7904562 | biostudies-literature

Immunosuppression in Honeybee Queens by the Neonicotinoids Thiacloprid and Clothianidin.

Project description:Queen health is crucial to colony survival of honeybees, since reproduction and colony growth rely solely on the queen. Queen failure is considered a relevant cause of colony losses, yet few data exist concerning effects of environmental stressors on queens. Here we demonstrate for the first time that exposure to field-realistic concentrations of neonicotinoid pesticides can severely affect the immunocompetence of queens of western honeybees (Apis mellifera L.). In young queens exposed to thiacloprid (200?µg/l or 2000?µg/l) or clothianidin (10?µg/l or 50?µg/l), the total hemocyte number and the proportion of active, differentiated hemocytes was significantly reduced. Moreover, functional aspects of the immune defence namely the wound healing/melanisation response, as well as the antimicrobial activity of the hemolymph were impaired. Our results demonstrate that neonicotinoid insecticides can negatively affect the immunocompetence of queens, possibly leading to an impaired disease resistance capacity.

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Acute effects of NOAEL-dose of neonicotinoid clothianidin on the gene expression profiles in cerebellum of male mice

Project description:The mechanisms of cognitive and learning effects of neonicotinoid pesticides were not fully understood. We examined the effects of acute exposure to a neonicotinoid pesticide, clothianidin (CLO) on gene expression profiles in the cellebellum of male mice at 10 weeks of age.

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Genetics of tolerance in honey bees to the neonicotinoid clothianidin.

Project description:The effects of neonicotinoid insecticides (NNIs) on honey bee health is intensely debated, with numerous studies showing negative effects of exposure, while others report no such effects. Understanding the cause of these differences is critical for developing evidence-based policy on the use of NNIs. We carried out experiments to study the genetic and molecular basis of NNI tolerance in honey bees, which may underlie the discrepancies observed in the literature. We discovered that worker survival post-exposure to an acute oral dose of clothianidin is heritable (H2=37.8%).

2023-03-01 | GSE216021 | GEO

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Developmental effects of neonatal exposure to NOAEL-dose of neonicotinoid clothianidin on the gene expression profiles of hippocampus in male mice

Project description:The mechanisms of developmental neurotoxicity of neonicotinoid pesticides were not fully understood. We examined the effects of neonatal exposure to a neonicotinoid pesticide, clothianidin (CLO) on gene expression profiles of the hippocampus in male mice at 3 or 10 weeks of age.

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Effects of sub-acute exposure of NOAEL-dose neonicotinoid clothianidin on the gene expression profiles of cerebral cortex and hippocampus in male mice

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2022-01-08 | GSE193245 | GEO

Effects of in utero and lactational exposure to low-dose neonicotinoid clothianidin on the gene expression profiles of the ovary in 3 weeks old mice

Project description:Neonicotinoid pesticides which were developed newly since the 1980s are chemically similar to nicotine. Prenatal and lactational exposure to neonicotinoid pesticides has been shown to cause reproductive toxicity in males, but not yet in female mice. We investigated effects of in utero and lactational exposure to a neonicotinoid pesticide, clothianidin (CLO) on gene expression profiles of the ovary in 3 weeks old mice.

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