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Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [RNA-Seq]

ABSTRACT: Background: Neonicotinoids, a widely used class of insecticide that used is agriculture to control insect’s population, are serious public concern. Objectives: In this study, we examined the capacity of a neonicotinoid, thiacloprid (thia) to induce transgenerational effects in the male mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE235213 | GEO | 2023/11/15

REPOSITORIES: GEO

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Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [MEDIP-seq]

Project description:Background: Neonicotinoids, a widely used class of insecticide that used is agriculture to control insect’s population, are serious public concern. Objectives: In this study, we examined the capacity of a neonicotinoid, thiacloprid (thia) to induce transgenerational effects in the male mice. Methods: The pregnant outbred Swiss female mice were exposed to thia at embryonic days E6.5 to E15.5 using “0”, and “6” mg/kg/day doses. The genome-wide analysis of DNA methylation analysis showed that differentially methylated regions (DMRs) are located at the promoters and enhancers of many important developmental genes. Notably, DMRs were largely overlapping the germ cell reprograming responsive gene (GRRs) and super-enhancer elements found to be active in ES cells, testis or brain.

2023-11-15 | GSE234861 | GEO

Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-15 | GSE235215 | GEO

Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice

Project description:Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice

| PRJNA985562 | ENA

Expression data in response to neonicotinoid insecticides

Project description:Neonicotinoid insecticides control crop pests based on their action as agonists at the insect nicotinic acetylcholine receptor which accepts chloropyridinyl- and chlorothiazolyl- analogs almost equally well. In some cases, these compounds have also been reported to enhance plant vigor and (a)biotic stress tolerance, independent of their insecticidal function. However, this mode of action has not been specifically defined. Using Arabidopsis thaliana, we show that the neonicotinoid compounds, imidacloprid (IMI) and clothianidin (CLO), via their 6-chloropyridinyl-3-carboxylic acid and 2-chlorothiazolyl-5-carboxylic acid metabolites, respectively, induce salicylic acid (SA) – associated plant responses. SA is a phytohormone best known for its role in plant defense against pathogens and as an inducer of systemic acquired resistance; however, it can also modulate abiotic stress responses. These neonicotinoids effect a similar global transcriptional response to that of SA including genes involved in (a)biotic stress response. Furthermore, similar to SA, IMI and CLO induce systemic acquired resistance resulting in reduced growth of a powdery mildew pathogen. The action of CLO induces the endogenous synthesis of SA via the SA biosynthetic enzyme ICS1, with ICS1 required for CLO-induced accumulation of SA, expression of the SA marker PR1, and fully enhanced resistance to powdery mildew. In contrast, the action of IMI does not induce endogenous synthesis of SA. Instead, IMI is further bioactivated to 6-chloro-2-hydroxypyridinyl-3-carboxylic acid, which is shown here to be a potent inducer of PR1 and inhibitor of SA-sensitive enzymes. Thus, via different mechanisms, these chloropyridinyl- and chlorothiazolyl- neonicotinoids induce SA responses associated with enhanced stress tolerance. response to neonicotinoid insecticides

2010-09-13 | E-GEOD-20188 | biostudies-arrayexpress

Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [MEDIP-seq]

Project description:Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [MEDIP-seq]

| PRJNA983361 | ENA

Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [RNA-Seq]

Project description:Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [RNA-Seq]

| PRJNA985566 | ENA

Stimulative effect of low-dose neonicotinoid pesticide, clothianidin on human neuroblastoma SH-SY5Y cells by disrupting intracellular signaling

Project description:Neonicotinoids are one of most widely used pesticides targeting nicotinic acetylcholine receptors (nAChRs) of insects and suspected to have adverse effects on mammals by laboratory testing on rodents. Recent epidemiological evidence revealed the increasing amounts of neonicotinoids detected from human samples, rising a critical question whether neonicotinoids affect to human health. We investigated potential effects of a neonicotinoid pesticide, clothianidin (CTD) on human neuroblastoma SH-SY5Y cells as a model of nervous systems of human. Transcriptional analyses using GeneChip system were also performed to understand the effects of CTD on global gene expression.

2019-02-06 | GSE126103 | GEO

Molecular bases of P450-mediated resistance to the neonicotinoid insecticide imidacloprid in the mosquito Ae. aegypti

Project description:Resistance to chemical insecticides including pyrethroids, the main insecticide class used against mosquitoes, led to a regain of interest for neonicotinoids. In this context, the present study aims at characterizing the molecular basis of neonicotinoid resistance in the mosquito Aedes aegypti. Resistance mechanisms were studied by combining transcriptomic and genomic data obtained from a laboratory strain selected at the larval stage for 30 generations with imidacloprid (Imida-R line). After thirty generations of selection, larvae of Imida-R line showed a 8-fold increased resistance to imidacloprid and a significant cross-tolerance to the pyrethroids permethrin and deltamethrin. Cross-resistance to pyrethroids was only observed in adults when larvae were previously exposed to imidacloprid suggesting a low but inducible expression of resistance alleles at the adult stage. Resistance of the Imida-R line was associated with a slower larval development time in females. Multiple detoxification enzymes were over-transcribed in larvae in association with resistance including the P450s CYP6BB2, CYP9M9 and CYP6M11 previously associated with pyrethroid resistance. Some of them together with their redox partner NADPH P450 reductase were also affected by non-synonymous mutations associated with resistance. Combining genomic and transcriptomic data allowed identifying promoter variations associated with the up-regulation of CYP6BB2 in the resistant line. Overall, these data confirm the key role of P450s in neonicotinoid resistance in Ae. aegypti and their potential to confer cross-resistance to pyrethroids, raising concerns about the use of neonicotinoids for resistance management in this mosquito species.

2021-05-07 | GSE174009 | GEO

Identification of molecular mechanisms associated with resistance to the neonicotinoid insecticide imidacloprid in the dengue vector Aedes aegypti

Project description:This microarray study aimed at comparing constitutive gene expression levels between an Aedes aegypti insecticide-resistant strain (Imida-R) selected at the larval stage with the neonicotinoid insecticide imidacloprid for 10 generations and the parental strain (Bora-Bora) susceptible to all insecticides. Strains comparison was performed at both larval (4th stage larvae) and adult (3 days-old adults females, non-blood fed) stages.

2012-03-31 | E-MTAB-616 | biostudies-arrayexpress

Expression data in response to neonicotinoid insecticides

2010-09-13 | GSE20188 | GEO