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Integrated analysis of the transcriptome-wide m6A methylome in gestational diabetes mellitus and healthy control placentas

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE154415 | GEO | 2021/12/31

REPOSITORIES: GEO

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Integrated analysis of the transcriptome-wide m6A methylome in gestational diabetes mellitus and healthy control placentas [meRIP-seq]

Project description:N6-methyladenosine (m6A) is the most prevalent modification in eukaryotic mRNA and potential regulatory functions of m6A have been shown by mapping the RNA m6A modification landscape. M6A modification in active gene regulation manifests itself as altered methylation profiles. However, the profiling of m6A modification and its potential role in gestational diabetes mellitus (GDM) has not yet been studied. In this work, placental samples were collected from GDM and control patients. MeRIP-seq was performed to identify differences in m6A methylation. Fragmented mRNAs were incubated for 2 h at 4 °C in the presence of 2 μg m6A antibodies (Synaptic Systems, 202003) in a 500 μl IP reaction system, and some of the fragments were used as input. RNA-seq libraries for m6A antibody-enriched mRNAs and input mRNAs were prepared using the KAPA Stranded mRNA-seq Kit (Illumina, CA, USA). Altered peaks of m6A-modified transcripts were primarily associated with mTOR signaling pathway, Notch signaling pathway, TGF-beta signaling pathway and so on. Our data provide novel information regarding m6A modification alterations in GDM and help our understanding of the pathogenesis of GDM.

2021-12-31 | GSE154413 | GEO

Integrated analysis of the transcriptome-wide m6A methylome in preeclampsia and healthy control placentas.

Project description: Not available

| S-EPMC7500358 | biostudies-literature

RNA sequence of gestational diabetes mellitus (GDM) and healthy control placentas

Project description:GDM is a multi-system disorder that is primarily characterised by new-onset hypertension accompanied by proteinuria during gestation. This disease is one of the leading causes of maternal and perinatal morbidity and mortality. In this work, placental samples were collected from GDM and control patients. RNA-seq was performed to identify differences in gene expression. Significantly differentially expressed genes between the GDM and control samples included 64 up-regulated and 296 down-regulated genes. This study may provide further insight in the mechanisms of GDM and function as preventive, predictive and therapeutic measures.

2021-12-31 | GSE154414 | GEO

Integrated analysis of the transcriptome-wide m6A methylome in gestational diabetes mellitus and healthy control placentas

Project description:Integrated analysis of the transcriptome-wide m6A methylome in gestational diabetes mellitus and healthy control placentas

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Integrated analysis of the transcriptome-wide m6A methylome in gestational diabetes mellitus and healthy control placentas [meRIP-seq]

Project description:Integrated analysis of the transcriptome-wide m6A methylome in gestational diabetes mellitus and healthy control placentas [meRIP-seq]

| PRJNA646213 | ENA

Integrated analysis of the transcriptome-wide m6A methylome in preeclampsia and healthy control placentas

Project description:N6-methyladenosine (m6A) is the most prevalent modification in eukaryotic mRNA and potential regulatory functions of m6A have been shown by mapping the RNA m6A modification landscape. M6A modification in active gene regulation manifests itself as altered methylation profiles. However, the profiling of m6A modification and its potential role in preeclampsia (PE) has not yet been studied. In this work, placental samples were collected from PE and control patients. MeRIP-seq was performed to identify differences in m6A methylatio. Altered peaks of m6A-modified transcripts were primarily associated with nitrogen compound metabolic process, positive regulation of vascular-associated smooth muscle cell migration, and endoplasmic reticulum organisation. The m6A hyper-methylated genes of Wnt/β-catenin signalling pathway, mTOR signalling pathway, and several cancer-related pathways may contribute to PE. Our data provide novel information regarding m6A modification alterations in PE and help our understanding of the pathogenesis of PE.

2020-08-24 | GSE143966 | GEO

RNAseq between gestational diabetes mellitus and healthy control groups

Project description:tsRNA profiles of gestational diabetes mellitus and healthy control groups were generated by deep sequencing using Illumina NextSeq 500.

2024-05-12 | GSE267259 | GEO

RNA sequence of gestational diabetes mellitus (GDM) and healthy control placentas

Project description:RNA sequence of gestational diabetes mellitus (GDM) and healthy control placentas

| PRJNA646212 | ENA

Integrated analysis of the transcriptome-wide m6A methylome in preeclampsia and healthy control placentas

Project description:Integrated analysis of the transcriptome-wide m6A methylome in preeclampsia and healthy control placentas

| PRJNA602367 | ENA

Genome wide methylation of cord blood from gestational diabetes mellitus

Project description:Genome wide DNA methylation profiling of cord blood cells obtained from gestational diabetes mellitus (GDM) pregnancies. The Illumina EPIC methylation beadchip array was used to obtain DNA methylation profiles across approximately 850,000 CpG dinucleotide methylation loci in DNA isolated from cord blood. Samples include 165 GDM subjects.

2021-06-23 | GSE122086 | GEO

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