Genomics

Dataset Information

0

LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice


ABSTRACT: Notch signaling primarily determines T-cell fate. However, the molecular mechanisms underlying the maintenance of T-lineage potential in pre-thymic progenitors remain unclear. Here, we established two Ebf1-deficient pro-B cell lines, with and without T-lineage potential. The latter expressed lower levels of Lmo2; their potential was restored via ectopic expression of Lmo2. Conversely, the CRISPR/Cas9-mediated deletion of Lmo2 resulted in the loss of the T-lineage potential. Introduction of Bcl2 rescued massive cell death of Notch-stimulated pro-B cells without efficient LMO2-driven Bcl11a expression but was not sufficient to retain their T-lineage potential. Pro-B cells without T-lineage potential failed to activate Tcf7 due to DNA methylation; Tcf7 transduction restored this capacity. Moreover, direct binding of LMO2 to the Bcl11a and Tcf7 loci was observed. Altogether, our results highlight LMO2 as a crucial player in the survival and maintenance of T-lineage potential in T-cell progenitors via the regulation of the expression of Bcl11a and Tcf7.

ORGANISM(S): Mus musculus

PROVIDER: GSE154472 | GEO | 2021/08/05

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-09-10 | GSE199696 | GEO
2021-09-12 | GSE162549 | GEO
2015-02-01 | E-GEOD-55237 | biostudies-arrayexpress
2023-10-24 | PXD030307 | Pride
2022-07-27 | PXD030301 | Pride
2012-09-11 | E-GEOD-40746 | biostudies-arrayexpress
2015-02-01 | GSE55237 | GEO
2009-01-13 | E-GEOD-13284 | biostudies-arrayexpress
2012-09-11 | GSE40746 | GEO
2016-03-26 | GSE79625 | GEO