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Gene regulation by m6A demethylase ALKBH5 in osteosarcoma [RNA-seq]

ABSTRACT: To identify target genes regulated by ALKBH5 in osteosarcoma, we silenced the expression of ALKBH5 in osteosarcoma cell line-143B and tested its effect on 143B transcriptome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE154528 | GEO | 2022/06/28

REPOSITORIES: GEO

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Gene regulation by m6A demethylase ALKBH5 in U2OS osteosarcoma

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Gene regulation by m6A demethylase ALKBH5 in osteosarcoma

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ALKBH5 is a New Mammalian RNA Demethylase

Project description:N6-methyladenosine (m6A) is the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Here we report ALKBH5 as a new mammalian demethylase that oxidatively removes the m6A modification in mRNA in vitro and inside cells. This demethylation activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles. Alkbh5-deficient male mice are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. In accordance with this defect, we have identified in mouse testes 1552 differentially expressed genes which cover broad functional categories and include spermatogenesis-related mRNAs involved in the p53 functional interaction network. We show that Alkbh5-deficiency impacts the expression levels of some of these mRNAs, supporting the observed phenotype. The discovery of this new RNA demethylase strongly suggests that the reversible m6A modification plays fundamental and broad functions in mammalian cells. RNA-seq in two cell types

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Genome-wide analysis of gene expression patterns by osteosarcoma tumor cells with different mtDNA genotypes

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Gene regulation by m6A demethylase ALKBH5 in osteosarcoma

Project description:This SuperSeries is composed of the SubSeries listed below.

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RNA demethylase ALKBH5 promotes tumorigenesis in multiple myeloma [m6A-seq]

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