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Regulation of dynamic pigment cell states at single-cell resolution

ABSTRACT: Pigment cells bear molecules with diverse physiological roles across phylogeny and are often under strict evolutionary selection. Thus, the mechanisms of regulating these cells, and the pigments within them, are of critical importance. Here, we explore the regulation of pigment cells in the purple sea urchin Strongylocentrotus purpuratus, an emerging model for understudied pigments at a molecular level. Sea urchins display a variety of pigments in both embryos and adults, but how the pigment cells form during development has not been resolved, nor has their breadth of biological significance been revealed. Known genes expressed by embryonic pigment cells are the transcriptional factor glial cells missing (gcm), and the enzymes pks1 and fmo1,2 and 3 (polyketide synthase1, flavin-dependent monooxygenase 1, 2, 3 respectively). In this study, we took advantage of single cell RNA-seq technology to interrogate the cell state of emerging pigment cells, and to test their diversity in fate and function.

ORGANISM(S): Strongylocentrotus purpuratus

PROVIDER: GSE155427 | GEO | 2020/07/31

REPOSITORIES: GEO

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