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Comparison of simple mutants gcn5 and hd1 and double mutant gcn5 hd1.

ABSTRACT: rs05-09_histone-acetylase - gcn5 - Different genes show an opposed expression profile in gcn5 compared to hd1. For these genes, the double mutant might show an expression similar to the wild type. - gcn5, hd1 or double mutant gcn5hd1 were grown on MS during 10 days (1.03 boyes stage). Keywords: gene knock out

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE15560 | GEO | 2009/04/11

SECONDARY ACCESSION(S): PRJNA115651

REPOSITORIES: GEO

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Comparison of simple mutants gcn5 and hd1 and double mutant gcn5 hd1.

Project description:rs05-09_histone-acetylase - gcn5 - Different genes show an opposed expression profile in gcn5 compared to hd1. For these genes, the double mutant might show an expression similar to the wild type. - gcn5, hd1 or double mutant gcn5hd1 were grown on MS during 10 days (1.03 boyes stage). Keywords: gene knock out 3 dye-swaps - CATMA arrays

2009-04-10 | E-GEOD-15560 | biostudies-arrayexpress

Transcription profiling of Arabidopsis gcn5 knockout mutants

Project description:Two gcn5 KO mutants were grown on MS during 10 days (1.03 boyes stage) : gcn5-1 and gcn5-2. Each mutant was compared to the wild type (Ws)

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Project description:Comparison of simple mutants gcn5 and hd1 and double mutant gcn5 hd1.

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Microarray comparative analysis of wild-type and hd1-3 mutant rice

Project description:Heading date1(Hd1) is a critical regulator controlling rice flowering time, which promotes flowering under short-day (SD) conditions and represses flowering under long-day (LD) conditions. In our previous study (Luan et al., 2009), we identified a rice mutant, hd1-3, in which the Hd1 gene was deficient due to several insertions/deletions in the coding region. To search for downstream genes regulated by Hd1, we performed microarray analysis of hd1-3 mutant and the wild-type Zhonghua11 under both SD and LD conditions. According to the microarray results, SDG712 gene was significantly downregulated in the hd1-3 mutant, indicating that SDG712 gene may acts downstream of Hd1, and may functions in rice flowering time regulation.

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PALB2 acetylation by KAT2A/B (GCN5/PCAF) in vitro

Project description:PArtner and Localizer of BRCA2 (PALB2) is essential to maintain genome stability in human cells. Upon DNA damage by double DNA strand breaks, PALB2 is required to repair DNA by homologous recombination. In undamaged conditions, PALB2 protects coding regions, by preventing DNA stress due to collisions between transcription and replication machineries. PALB2 associates with chromatin, which is essential to fulfill its function in genome stability maintenance, however molecular mechanisms regulating PALB2 chromatin association remain unknown. In our previous published study describing the KAT2A/B(GCN5/PCAF)-acetylome (Fournier et al., Nat.Comm. 2016, doi: 10.1038/ncomms13227) we have identified PALB2 as an acetylated protein target of the acetyltransferases KAT2A (GCN5) and KAT2B (PCAF) in vivo. KAT2A/B-acetylated sites of PALB2 were mapped within its DNA/Chromatin association domain. In this current study, we have conducted in vitro acetyltransferase (AT) assays, by mixing purified recombinant GST-tagged PALB2 full-length (FL) and PALB2 fragment P2.2 which associates with DNA/chromatin (from residues 295-610), with Flag-PCAF, Flag-GCN5 and Flag-GCN5 catalytic mutant (mut), followed by proteomics analysis to map PALB2 acetylated lysines by KAT2A(GCN5) and KAT2B(PCAF) in vitro. PALB2 FL and P2.2 alone, or PALB2 FL and P2.2 mixed with GCN5 catalytic mutant, were used as negative controls.

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Bacillus cereus strain:HD1

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Gcn5 and PCAF negatively regulate interferon β production through HAT-independent inhibition of TBK1 [RNA-Seq]

Project description:Gcn5/PCAF double knockout (dKO) leads to loss of the global H3K9ac. RNA-Seq was performed to define the changes of gene expression in response to Gcn5/PCAF deletion and H3K9ac loss PCAF-/-;Gcn5f/D MEFs were infected with retroviral Cre to delete Gcn5 to generate Gcn5/PCAF dKO cells, followed by RNA-Seq analysis using spike-in RNA as controls

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Gcn5 and PCAF negatively regulate interferon β production through HAT-independent inhibition of TBK1 [RNA-Seq]

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Comparison of gene regulation by Gcn5 under KCl stress in S. cerevisiae and S. pombe

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Comparison of gene regulation by Gcn5 under KCl stress in S. cerevisiae and S. pombe

Project description:This is parallel comparison of gene regulation by Gcn5 in evolutionarily divergent yeasts S. pombe and S. cerevisiae. Our study showed Gcn5 is required for a similar spectrum of stress responses in both organisms, including the response to KCl. This DNA microarray studies is to find conserved or diverged gene regulation pattern under KCl stress condition in the two yeasts. There are 6 subsets in total, 3 subsets in each evolutionarily divergent yeasts including the following: 1 gcn5 mutant compared to wt under rich medium without treatment, 2 gcn5 mutant compared to wild type both under KCl treatment, 3 wild type strains under KCl treatment compared to wild type without treatment. Keywords: stress response, Gcn5, Comparative genomics, Transcriptional co-activator Gene expression profiling experiment in which gene expression in logarithmically growing cultures of test groups was compared to that of the control groups in rich medium at 25C. There are 6 subsets in total, 3 subsets in each yeast including the following: 1 gcn5 mutant compared to wt under rich medium without treatment, 2 gcn5 mutant compared to wild type both under KCl treatment, 3 wild type strains under KCl treatment compared to wild type without treatment. RNA was extracted and subjected to cDNA expression profiling analysis using the established protocols (Xue et al, 2004). Independent RNA preparations were used to hybridize to microarray slides with dye swap. Data normalized with 'Lowess' per chip per spot normalization.

2010-06-24 | E-GEOD-5218 | biostudies-arrayexpress

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