Project description:rs05-09_histone-acetylase - gcn5 - Different genes show an opposed expression profile in gcn5 compared to hd1. For these genes, the double mutant might show an expression similar to the wild type. - gcn5, hd1 or double mutant gcn5hd1 were grown on MS during 10 days (1.03 boyes stage). Keywords: gene knock out 3 dye-swaps - CATMA arrays
Project description:Presence of an inhibitor of the cytochrome pathway (antimycine A). This treatment induces the expression of a gene coding for an alternative oxydase (AOX1a) and one of the two genes coding for apocytochrome c (CYTc-a).
Project description:The homozygous arabidopsis line 9S9 overproducing AtCCME-HIS (At3g1790), a mitochondrial heme chaperon involved in c-type cytochrome maturation will be compared with the corresponding wild type (Wassilievskjia).
Project description:GCN5 plays an essential role in chromatin modification and transcriptional regulation, its mode of action is not understood. It is therefore crucial to determine what are the sites targeted by GCN5 in the Arabidopsis genome to decipher the regulatory mechanisms in which it is involved. Array technologies have been used in recent years to determine, at the scale of whole genomes, the sites bound in vivo by chromatin-associated proteins (Hanlon and Lieb 2004) or targeted by chromatin-modifying enzymes (Millar and Grunstein 2006; Zhang et al. 2006). In this study we took advantage of the chromatin immunoprecipitation (ChIP) technique coupled with hybridization to a novel Arabidopsis promoter array to show that GCN5 is recruited to a large number of transcriptionally active promoters.
Project description:During a compatible interaction, root-knot nematodes (Meloidogyne spp.) induce the redifferentiation of root cells into multinucleate nematode feeding cells giant cells. These hypertrophied cells result from repeated nuclear divisions without cytokinesis, are metabolically active and present features typical of transfer cells. Hyperplasia of the surrounding cells leads to formation of the typical root gall. We investigate here the plant response to root-knot nematodes.
Project description:We want to know which genes are overexpressed or down regulated in Arabidopsis plants transformed with the sunflower hahb-4 homeodomain transcription factor with respect to non transformed ones in control and water stress conditions. This gene, hahb4, confers drought tolerance to transgenic arabidopsis plants. Aa water stress was applied to transgenic and non transformed three weeks old arabidopsis plants control and overexpressing line
Project description:Inhibition of cellulose synthesis by chemical inhibitors or in a mutant background leads to rapid inhibition of cell elongation. This inhibition appears to be an active process, which involves feedback signalling from the cell wall. We have isolated two loci THE1 and THE2, which are identified by mutations that partially suppress the dark-grown hypocotyl phenotype in a mutant background for cellulose synthase catalytic subunit CESA6_PROCUSTE1. THE1 encodes a receptor kinase and may play a role in this feedback signalling process. To identify genes that are regulated by THE1 and THE2, we compared the transcript profiles of 5 day-old dark-grown seedlings of the1-1_prc1-1 with prc1-1 ; the1-3_prc1-8 with prc1-8 ; prc1-8 or the1-3 with WS and the2-1_prc1-1 with prc1-1.
Project description:Identification of molecular target of MGO3 genes. 2 first leaves of mgo3-2 mutant or wild-type plants (WS ecotype) were harvested from plants grown during 2 weeks in vitro on MS medium in long days conditions. 3 repeats have been done for each samples