Transcriptomics

Dataset Information

0

Transcriptional effects of MAF- and MAFB-siRNAs on M-CSF-derived macrophages


ABSTRACT: Analysis of the role of transcriptions factors MAF and MAFB on the phenotypic profles of human M-CSF-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) for 7 days in the presence of 10 ng/ml M-CSF to generate M-CSF-polarized macrophages (M-MØ). Macrophages were differentiated from peripheral blood monocytes from 3 healthy donors with M-CSF (M-MØ) to generate anti-inflammatory M-MØ. Macrophages were transfected with either Control siRNA or MAFB-specific siRNA or MAF-specific siRNA for 24h and global gene expression was analysed by RNA-Seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE155719 | GEO | 2020/12/09

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-07-06 | GSE205719 | GEO
2022-03-24 | GSE188278 | GEO
2023-07-01 | GSE181249 | GEO
2024-02-08 | GSE224131 | GEO
2022-06-08 | GSE180897 | GEO
2023-12-24 | GSE207840 | GEO
2020-12-09 | GSE156921 | GEO
2022-06-08 | GSE181250 | GEO
2023-07-14 | GSE156696 | GEO
2022-03-24 | GSE156783 | GEO