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RNA Sequencing facilitates quantitative analysis of differently expressed genes with GA or SKN treatment in rheumatoid fibroblast-like synoviocytes

ABSTRACT: To explore downstream gene expression profile of PKM2,we evaluated the transcriptome of GA- or SKN-treated RA FLSs compared to untreated control using RNA sequencing analysis. The data were analyzed for differential expression with a nominal P<0.05. GA Treatment induced downregulation of 46 genes and upregulation of 58 genes compared to DMSO treatment. SKN treatment resulted in downregulation of 14 genes and upregulation of 27 genes compared to DMSO treatment.A Venn diagram showed the overlap of the significantly expressed genes between GA and SKN treatment. We found that in the overlap of both GA and SKN treatment, STAT5A and ARHGAP11A were significantly downregulated, and THAP6 and AHRR were significantly upregulated.

ORGANISM(S): Homo sapiens

PROVIDER: GSE155766 | GEO | 2020/08/06

REPOSITORIES: GEO

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SKN-1-dependent oxidative stress response in C. elegans

Project description:Oxidative stress may play a role in normal aging. SKN-1 is a transcription factor necessary for intestine development in Caenorhabditis elegans, which also regulates the response to oxidative stress post-embryonically. Using DNA microarrays, we found that oxidative stress induces the antioxidant response, the heat shock response, and detoxification genes, while the expression of genes involved in homeostasis, development, and reproduction were decreased. Both up-regulated and down-regulated genes can be wholly, partially, or not at all dependent on SKN-1 action. However, induction of the heat shock response by oxidative stress was not affected by SKN-1 removal. Keywords: stress response

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RNA Sequencing facilitates quantitative analysis of differently expressed genes with GA or SKN treatment in rheumatoid fibroblast-like synoviocytes

Project description:RNA Sequencing facilitates quantitative analysis of differently expressed genes with GA or SKN treatment in rheumatoid fibroblast-like synoviocytes

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2011-02-18 | E-GEOD-25810 | biostudies-arrayexpress

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TRX-1 Regulates SKN-1 Nuclear Localization Cell Non-Autonomously in Caenorhabditis elegans

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