Genomics

Dataset Information

0

M6A methylation regulates the fate of endogenous retrovirus transcripts [CUT & RUN]


ABSTRACT: Using a genome-scale CRISPR knockout screening in mouse embryonic stem cells, we identify m6A RNA methylation as an important regulatory component that restricts the activity of endogenous retroviruses of the IAP (intracisternal A-particles) family. The m6A methylation of IAP mRNAs occurs on their 5’ end, is catalyzed by the complex of methyltransferase-like (METTL)3/METTL14 proteins whose depletion, along with other complex subunits, namely WTAP and ZC3H13, leads to increased IAP transcript abundance. Using auxin-dependent degron, we show that rapid removal of METTL3, METTL14 and ZC3H13 further increases IAP RNA abundance in a progressive and reversible fashion. Finally, we demonstrate that m6A RNA reduces the stability of IAP transcripts

ORGANISM(S): Mus musculus

PROVIDER: GSE156007 | GEO | 2020/08/11

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-04-13 | GSE145615 | GEO
2020-04-13 | GSE145315 | GEO
2020-04-13 | GSE145309 | GEO
2018-03-14 | GSE94148 | GEO
2019-02-21 | GSE110320 | GEO
2018-03-07 | GSE102493 | GEO
2024-08-14 | GSE268352 | GEO
2022-06-23 | PXD028456 |
2015-02-27 | E-GEOD-56010 | biostudies-arrayexpress
2023-08-18 | PXD036899 | Pride