The effect of lncRNA PART1 knockdown on gene expression in HCC1806 and HCC1395 cells
Ontology highlight
ABSTRACT: PART1 was knocked down down using GapmeRs specific to PART1. The breast cancer cells used in this study were confirmed to have PART1 expression by qPCR and knockdown of PART was confirmed by qPCR.
Project description:PART1 was knocked down down using GapmeRs specific to PART1. The breast cancer cells used in this study were confirmed to have PART1 expression by qPCR and knockdown of PART was confirmed by qPCR.
Project description:The expression of lncRNA PART1 was found to be significantly upregulated in platinum-sensitive patients with ovarian cancer, and this upregulation was positively correlated with a favorable prognosis. Cellular experiments demonstrated that inhibition of PART1 led to cellular senescence and increased resistance to cisplatin and PARP inhibitors. Thus, LncRNA PART1 is a novel target for overcoming resistance to PARP inhibitors in ovarian cancer.
Project description:PAX6 is an evolutionary conserved transcription factor important for normal development of the CNS, eye and pancreas. Its role in cancer is however poorly understood as both tumor suppressive- and oncogenic properties have been reported. We generated a pancreatic adenocarcinoma cell line (HPAFII) were PAX6 was knocked down by the use of short hairpin RNA. To generate a control cell line scrambled short hairpin RNA was used. Successful knock down of PAX6 was confirmed by RT-qPCR and Western blot. RNA were harvested from shPAX6, shControl and untransfected HPAFII cells, and used in gene expression microarray to look for differences in gene expression when PAX6 was knocked down.
Project description:We have performed a transcriptomics study in which we first transfected LNA GapmeRs against DR5-AS lncRNA to silence it in HeLa cells. Total RNA was isolated from control as well as transfected cells and silencing was confirmed by qPCR. Total RNAs were subjected to deep-sequencing to identify differentially expressed mRNAs. We then took advantage of bioinformatic tools to identify which pathways are affected by DR5-AS knock-down.
Project description:Tissue-resident macrophages comprise heterogeneous populations with unique functions and distinct gene expression signatures. While it has been established that they mostly originate from embryonic progenitors, the signals inducing a characteristic tissue-specific differentiation program remain unknown. Here we identify PPARγ as the crucial transcription factor determining perinatal alveolar macrophage (AM) development and identity. Development of the fetal monocyte derived AM precursor was largely abrogated in CD11c-Cre/Ppargfl/fl mice. To reveal the underlying changes in gene expression, we performed microarray analysis of sorted WT and KO AM and pre-AM from 3 different timepoints. Part1: d2 and d11 sorts & array