Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of TET deletion on the global DNA methylation status of osteoclasts (RNA-Seq)

ABSTRACT: Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain incompletely understood. We performed RNA-seq analysis to investigate gene expression in osteoclasts derived from wild-type and Tet2Rank–/–; Tet3aRank+/– mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE156129 | GEO | 2021/11/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Effect of TET deletion on the global DNA methylation status of osteoclasts (MeDIP-Seq)

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain incompletely understood. We performed MeDIP-seq analysis to investigate DNA methylation in osteoclasts derived from wild-type and Tet2Rank–/–; Tet3aRank+/– mice.

2021-11-29 | GSE156128 | GEO

Effect of Dnmt3a deletion on the global DNA methylation status of osteoclasts

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain to be elucidated. We performed MBD-seq analysis to investigate DNA methylation in osteoclasts derived from wild-type and osteoclast-specific Dnmt3a knockout mice. Examination of DNA methylation in 2 cell types.

2015-02-23 | E-GEOD-59388 | biostudies-arrayexpress

Effect of Dnmt3a deletion on the global DNA methylation status of osteoclasts

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain to be elucidated. We performed MBD-seq analysis to investigate DNA methylation in osteoclasts derived from wild-type and osteoclast-specific Dnmt3a knockout mice.

2015-02-23 | GSE59388 | GEO

Expression data from wild-type control and Dnmt3a knockout osteoclast

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain to be elucidated. We performed microarray analysis to investigate gene expression in osteoclasts derived from wild-type and Dnmt3aknockout mice. In vitro osteoclast culture were performed using wild-type control and Dnmt3a knockout bone marrow-derived monocyte/macrophage precursor cells.

2015-02-23 | E-GEOD-59387 | biostudies-arrayexpress

Expression data from wild-type control and Dnmt3a knockout osteoclast

2015-02-23 | GSE59387 | GEO

Expression data from cultured macrophages and osteoclasts

Project description:Osteoclastogenesis is induced by the stimulation of RANKL. In the early stage of osteoclast differentiation, the osteoclast progenitor cells are primed by M-CSF, following a tightly controlled genetic program where specific sets of genes are up-regulated by RANKL. Some of them, for instance, control differentiation, cell-cell fusion and bone resorption. We used microarrays to detail the global program of gene expression underlying osteoclastogenesis and identified various up-regulated genes during this process. Macrophages and osteoclasts were cultured for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of macrophages and osteoclasts in order to increase the temporal resolution of expression profiles. To that end, mouse bone marrow cells were cultured in the presence of M-CSF for three days and harvested as macrophage and oseteoclast common progenitor cells. Then common progenitor cells were further cultured in the presence of M-CSF alone for macrophages and M-CSF plus RANKL for osteoclasts, respectively.

2010-03-22 | E-GEOD-20850 | biostudies-arrayexpress

Effect of TET deletion on the global DNA methylation status of osteoclasts

Project description:Effect of TET deletion on the global DNA methylation status of osteoclasts

| PRJNA656813 | ENA

A novel method to efficiently differentiate human osteoclasts from blood-derived monocytes

Project description:Osteoclasts are the tissue-specific macrophage population of the bone and unique in their bone-resorbing activity. Hence, they are fundamental for bone physiology in health and disease. However, efficient protocols for the isolation and study of primary human osteoclasts are scarce. In this study, we aimed to establish a protocol, which enables the efficient differentiation of functional human osteoclasts from monocytes. To this end, human monocytes were isolated through a double-density gradient from healthy donor blood. The differentiation to macrophages was performed either in cell culture dishes or in Teflon-coated bags. The impact of these two distinct differentiation protocols on the gene expression profile of macrophages was assessed via RNA-Sequencing. The macrophages were then further differentiated to terminal osteoclasts through the addition of Receptor Activator of NF-κB Ligand (RANKL). The yield and function of multinuclear osteoclasts was significantly increased upon initial differentiation of monocytes to macrophages in Teflon-coated bags. Our study has established a novel protocol for the isolation of primary human osteoclasts that improves osteoclastogenesis in comparison to the conventionally used cultivation approach.

2023-12-04 | GSE248902 | GEO

Sex differential gene expression in macrophages and osteoclasts

Project description:To determine differences in gene expression during osteoclastogenesis, we performed sequencing on RNA extracted from MCSF-differentiated bone marrow macrophages and MCSF+RANKL differentiated osteoclasts from male and female C56Bl/6 mice

2022-11-03 | GSE216929 | GEO

Effect of TET deletion on the global DNA methylation status of osteoclasts (MeDIP-Seq)

Project description:Effect of TET deletion on the global DNA methylation status of osteoclasts (MeDIP-Seq)

| PRJNA656818 | ENA