Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from wild-type control and Dnmt3a knockout osteoclast

ABSTRACT: Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain to be elucidated. We performed microarray analysis to investigate gene expression in osteoclasts derived from wild-type and Dnmt3aknockout mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE59387 | GEO | 2015/02/23

SECONDARY ACCESSION(S): PRJNA255230

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from wild-type control and Dnmt3a knockout osteoclast

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain to be elucidated. We performed microarray analysis to investigate gene expression in osteoclasts derived from wild-type and Dnmt3aknockout mice. In vitro osteoclast culture were performed using wild-type control and Dnmt3a knockout bone marrow-derived monocyte/macrophage precursor cells.

2015-02-23 | E-GEOD-59387 | biostudies-arrayexpress

Effect of Dnmt3a deletion on the global DNA methylation status of osteoclasts

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain to be elucidated. We performed MBD-seq analysis to investigate DNA methylation in osteoclasts derived from wild-type and osteoclast-specific Dnmt3a knockout mice. Examination of DNA methylation in 2 cell types.

2015-02-23 | E-GEOD-59388 | biostudies-arrayexpress

Effect of Dnmt3a deletion on the global DNA methylation status of osteoclasts

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain to be elucidated. We performed MBD-seq analysis to investigate DNA methylation in osteoclasts derived from wild-type and osteoclast-specific Dnmt3a knockout mice.

2015-02-23 | GSE59388 | GEO

Effect of TET deletion on the global DNA methylation status of osteoclasts (RNA-Seq)

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain incompletely understood. We performed RNA-seq analysis to investigate gene expression in osteoclasts derived from wild-type and Tet2Rank–/–; Tet3aRank+/– mice.

2021-11-29 | GSE156129 | GEO

Effect of TET deletion on the global DNA methylation status of osteoclasts (MeDIP-Seq)

Project description:Epigenetic regulation is a fundamental mechanism mediating various cellular processes. However, epigenetic mechanisms in osteoclastogenesis remain incompletely understood. We performed MeDIP-seq analysis to investigate DNA methylation in osteoclasts derived from wild-type and Tet2Rank–/–; Tet3aRank+/– mice.

2021-11-29 | GSE156128 | GEO

Gene microarray screening to identify glycosylation genes that are regulated during the formation of osteoclast cells

Project description:Bone remodeling is a tightly regulated process that engages degradation and biogenesis of the bone matrix. The process is controlled by two major cell types, bone forming cells-osteoblasts and bone-degrading cells-osteoclasts. We are interested in the bone-resorption mechanism mediated by osteoclasts and wish to identify glycosylation genes that are regulated during the formation of osteoclast cells and determine the function of glycosylation and glycan-binding proteins in the osteoclastogenesis. We propose to examine the gene expression patterns that are altered during the osteoclastogenesis using mouse glyco-chips and RNA samples isolated from osteoclast precursors and mature osteoclasts prepared from mouse bone marrows. 6 chips are requested for the analysis. RNA preparations from mouse bone marrow MG2, MG4, MG6 (mature osteoclasts) and MG1, MG3, MG5 osteoclasts precursors (control) were sent to the Microarray Core (E). The RNA was amplified, labeled, and hybridized to the GLYCOv3 microarrays.

2011-04-29 | E-GEOD-28937 | biostudies-arrayexpress

The HIV co-receptor CCR5, regulates the cellular architecture of osteoclast

Project description:C-C chemokine receptor 5 (CCR5) is a co-receptor of HIV. Its ligand, CCL5 significantly augmented the number of wild-type osteoclasts but not Ccr5-deficient cells, indicating that CCL5 enhanced RANKL-induced osteoclastogenesis through CCR5. To investigate the changes in the transcriptional signatures induced by CCL5 in osteoclastogenesis, cultured osteoclasts at pOC stages were incubated with or without rmCCL5 for 2 days, and then subjected for RNA sequencing.

2017-11-15 | GSE106259 | GEO

Inhibition of DOT1L increases osteoclast fusion and resorption and aggravates bone loss

Project description:Osteoclasts are absorptive cells and play a critical role in homeostatic bone remodeling and pathological bone resorption. Emerging evidence suggests an important role for epigenetic regulation of osteoclastogenesis. In this study, we investigated the role of DOT1L, which regulates gene expression epigenetically by histone H3K79 methylation during osteoclast formation. DOT1L and H3K79me2 levels were upregulated during osteoclast differentiation. Small molecule inhibitor- (EPZ5676 or EPZ004777) or short hairpin RNA-mediated reduction in DOT1L expression promoted osteoclast differentiation and resorption. DOT1L inhibition also increased osteoclast area and accelerated bone mass reduction in a mouse ovariectomy (OVX) model of osteoporosis. DOT1L inhibitors did not alter osteoblast differentiation in vitro and in vivo. Proteomics data, together with bioinformatics analysis, revealed that DOT1L inhibition altered reactive oxygen species (ROS) generation, autophagy activation, and cell fusion-related protein expression. ROS generation increased, and autophagy activation and cell migration ability enhancement were verified subsequently by flow cytometry and transwell migration assays. DOT1L inhibition increased NFATc1 nuclear translocation and NF-κB activation and strengthend osteoclast fusion and expression of resorption-related protein CD9, and MMP9 in osteoclasts derived from RAW264.7. Our findings support a new mechanism of DOT1L-mediated H3K79me2 epigenetic regulation of osteoclast differentiation, implicating DOT1L as a new therapeutic target for osteoclast dysregulation-induced disease.

2018-01-24 | PXD006448 | Pride

Identification of DNA methylation changes in monocytes to osteoclast differentiation

Project description:Conversion of monocytes to osteoclasts is a unique terminal differentiation process within the hematopoietic system involving differentiation and massive cell fusion. Here we focused on DNA methylation changes during osteoclastogenesis. Hypermethylation and hypomethylation changes took place in several thousand genes, including all relevant functional categories in osteoclast differentiation and function. Comparison between the DNA methylation levels of CD14+ monocytes and derived osteoclast from 3 female donors. Bisulphite converted DNA from the 6 samples was hybridised to the Illumina Infinium 450k Human Methylation Beadchip

2013-08-30 | E-GEOD-46648 | biostudies-arrayexpress

Identification of DNA methylation changes in monocytes to osteoclast differentiation

2013-08-30 | GSE46648 | GEO