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CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators I

ABSTRACT: Cellular RNA is decorated with over 160 types of chemical modifications. Many modifications in mRNA, including m6A and m5C, have been associated with critical cellular functions under physiological and/or pathological conditions. To understand the biological functions of these modifications, it is vital to identify the regulators that could modulate the modification rate. However, a high-throughput method for unbiased screening of these regulators is so far lacking. Here, we report such a method combining pooled CRISPR screen and reporters with RNA modification readout, termed CRISPR integrated gRNA and reporter sequencing (CIGAR-seq).

ORGANISM(S): Homo sapiens

PROVIDER: GSE157366 | GEO | 2020/11/04

REPOSITORIES: GEO

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