Transcriptomics

Dataset Information

0

The RyfA small RNA serves as a master regulator of stress responses and virulence in uropathogenic Escherichia coli


ABSTRACT: Urinary tract infections (UTIs) are a very common bacterial infectious disease in humans, and uropathogenic Escherichia coli (UPEC) are the most frequent cause of UTIs. During infection, UPEC must cope with a variety of stressful conditions in the urinary tract. Here, we demonstrated that the small RNA (sRNA) RyfA of UPEC strains was required for resistance to oxidative and osmotic stresses. Inactivation of ryfA in UPEC strain CFT073 decreased urinary tract colonization in CBA/J mice and the ryfA mutant also had reduced production of type 1 and P fimbriae, which are known to be important for UTI. Transcriptomic analysis of the ryfA mutant showed changes in expression of genes associated with general stress responses, metabolism, biofilm formation and genes coding for cell surface proteins. Furthermore, loss of ryfA also reduced UPEC survival in human macrophages. Thus, ryfA plays a key regulatory role in UPEC adaptation to stress, that contributes to UTI and survival in macrophages.

ORGANISM(S): Escherichia coli

PROVIDER: GSE157450 | GEO | 2021/06/23

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-10-26 | GSE160038 | GEO
2016-01-08 | E-GEOD-43376 | biostudies-arrayexpress
2019-10-29 | GSE128997 | GEO
2016-01-08 | GSE43376 | GEO
2021-11-06 | GSE188170 | GEO
2012-04-05 | GSE28399 | GEO
2012-04-04 | E-GEOD-28399 | biostudies-arrayexpress
2019-05-18 | GSE131382 | GEO
2022-06-09 | PXD032049 | Pride
2018-08-10 | GSE96042 | GEO