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Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE157532 | GEO | 2020/10/31

REPOSITORIES: GEO

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Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures.

Project description:Zika virus (ZIKV) is a mosquito-borne virus, which can cause brain abnormalities in newborns, including microcephaly. MicroRNAs (miRNAs) are small non-coding RNAs, which post- transcriptionally regulate gene expression. They are involved in various processes including neurological development and host responses to viral infection, but their potential role in ZIKV pathogenesis remains poorly understood. MiRNAs can be incorporated into extracellular vesicles (EVs) and mediate cell-to-cell communication. While it is well known that in viral infections EVs carrying miRNAs can play a crucial role in disease pathogenesis, ZIKV effects on EV-delivered miRNAs and their contribution to ZIKV pathogenesis have not been elucidated. In the present study, we profiled intracellular and EV-derived miRNAs by next generation sequencing and analyzed the host mRNA transcriptome of neural stem cells during infection with ZIKV Uganda and French Polynesia strains. We identified numerous miRNAs, including miR-4792, which were dysregulated at the intracellular level and had altered levels in EVs during ZIKV infection. Integrated analyses of differentially expressed genes and miRNAs showed that ZIKV infection had an impact on processes associated with neurodevelopment and oxidative stress. Our results provide insights into the roles of intracellular and EV-associated host miRNAs in ZIKV pathogenesis.

| S-EPMC7693339 | biostudies-literature

Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures [array]

Project description:We used whole genome expression profiling to analyze the effects of Zika virus (ZIKV) strains Uganda and French Polynesia on the mRNA transcriptome of induced pluripotent stem cell-derived human neural stem cells (long-term self-renewing neuroepithelial-like stem (lt-NES®) cells) - particularly to identify differentially expressed genes which are associated with microcephaly phenotype.

2020-10-31 | GSE157530 | GEO

Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures [miRNA-Seq]

Project description:We used next generation sequencing to analyze the effects of Zika virus (ZIKV) strains Uganda and French Polynesia on intracellular and extracellular vesicle (EV) derived miRNA profiles of induced pluripotent stem cell-derived human neural stem cells (long-term self-renewing neuroepithelial-like stem (lt-NES®) cells) - particularly to identify dysregulated miRNAs which are associated with the microcephaly phenotype.

2020-10-31 | GSE157528 | GEO

Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures

Project description:Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures

| PRJNA661574 | ENA

Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures [array]

Project description:Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures [array]

| PRJNA661577 | ENA

Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures [miRNA-Seq]

Project description:Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures [miRNA-Seq]

| PRJNA661578 | ENA

Zika infected neural stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-06-18 | GSE113640 | GEO

Epigenetic abnormality due to maternal Zika virus infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-06-30 | GSE164783 | GEO

Zika virus infection dysregulates human neural stem cell growth and inhibits differentiation into neuroprogenitor cells.

Project description:The current outbreak of Zika virus-associated diseases in South America and its threat to spread to other parts of the world has emerged as a global health emergency. A strong link between Zika virus and microcephaly exists, and the potential mechanisms associated with microcephaly are under intense investigation. In this study, we evaluated the effect of Zika virus infection of Asian and African lineages (PRVABC59 and MR766) in human neural stem cells (hNSCs). These two Zika virus strains displayed distinct infection pattern and growth rates in hNSCs. Zika virus MR766 strain increased serine 139 phosphorylation of histone H2AX (?H2AX), a known early cellular response proteins to DNA damage. On the other hand, PRVABC59 strain upregulated serine 15 phosphorylation of p53, p21 and PUMA expression. MR766-infected cells displayed poly (ADP-ribose) polymerase (PARP) and caspase-3 cleavage. Interestingly, infection of hNSCs by both strains of Zika virus for 24?h, followed by incubation in astrocyte differentiation medium, induced rounding and cell death. However, astrocytes generated from hNSCs by incubation in differentiation medium when infected with Zika virus displayed minimal cytopathic effect at an early time point. Infected hNSCs incubated in astrocyte differentiating medium displayed PARP cleavage within 24-36?h. Together, these results showed that two distinct strains of Zika virus potentiate hNSC growth inhibition by different mechanisms, but both viruses strongly induce death in early differentiating neuroprogenitor cells even at a very low multiplicity of infection. Our observations demonstrate further mechanistic insights for impaired neuronal homeostasis during active Zika virus infection.

| S-EPMC5682681 | biostudies-other

Differential Responses of Human Fetal Brain Neural Stem Cells to Zika Virus Infection.

Project description:Zika virus (ZIKV) infection causes microcephaly in a subset of infants born to infected pregnant mothers. It is unknown whether human individual differences contribute to differential susceptibility of ZIKV-related neuropathology. Here, we use an Asian-lineage ZIKV strain, isolated from the 2015 Mexican outbreak (Mex1-7), to infect primary human neural stem cells (hNSCs) originally derived from three individual fetal brains. All three strains of hNSCs exhibited similar rates of Mex1-7 infection and reduced proliferation. However, Mex1-7 decreased neuronal differentiation in only two of the three stem cell strains. Correspondingly, ZIKA-mediated transcriptome alterations were similar in these two strains but significantly different from that of the third strain with no ZIKV-induced neuronal reduction. This study thus confirms that an Asian-lineage ZIKV strain infects primary hNSCs and demonstrates a cell-strain-dependent response of hNSCs to ZIKV infection.

| S-EPMC5355569 | biostudies-literature

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