Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE157535 | GEO | 2024/11/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

Project description:Metastasis is the leading cause of cancer-related mortality. Cancer stem cells contribute to metastasis in the murine colon cancer models, but the underlying mechanisms are unclear. Here we report a Wnt ligand, Dickkopf2 (DKK2) is essential for colorectal cancer stemness. Genetic depletion of Dkk2 in intestinal epithelial or stem cells reduced tumorigenesis as well as expression of the stem cell marker gene Lgr5 in a model of colitis-associated cancer. Mechanistically, DKK2 activates c-Src followed by increased LGR5 expressing stem cells in colorectal cancer through degradation of HNF4α1. Splenic injection of DKK2-deficient cancer organoids into C57BL/6 mice resulted in a significant reduction of liver metastases compared to the control cancer organoids in spite of the presence of oncogenic mutations in Apc, Kras and Tp53 genes. These findings suggest that DKK2 is required for stemness of colorectal cancer cells, which in turn contributes to metastasis.

2024-11-11 | GSE157529 | GEO

Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

Project description:Metastasis is the leading cause of cancer-related mortality. Paneth cells provide stem cell niche factors in the homeostatic condition, but the underlying mechanisms of cancer stem cell niche development are unclear. Here we report that Dickkopf-2 (DKK2) is essential for Paneth-like cell generation during the metastasis of colon cancer. Splenic injection of DKK2-knockout (KO) cancer organoids into C57BL/6 mice resulted in a significant reduction of liver metastases compared to the control cancer organoids in spite of the presence of carcinogenic mutations in Apc, Kras and Tp53 genes. RNA-seq analysis of DKK2 KO organoids showed reduction of Paneth cell marker genes such as Lysozymes and Defensins. Ingenuity pathway analysis (IPA) of the RNA-seq data and assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) suggested HNF4A as a downstream target of DKK2. Chromatin immunoprecipitation followed by sequencing (Chip-seq) analysis showed that HNF4A binds to the open chromatin regions of Sox9, a well-known transcription factor for Paneth cell differentiation. Reduction of Paneth-like cells in the absence of DKK2 has been confirmed in the murine metastatic model of colon cancer using flow cytometry, immunohistochemistry and single cell RNA-seq. These data suggest the necessity of DKK2 in Paneth-like cell generation. Thus, DKK2 contributes to stem cell niche development during the metastatic progression of colon cancer.

2024-11-11 | GSE277510 | GEO

Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties [RNA-seq]

2024-11-11 | GSE157531 | GEO

Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

Project description:Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

| PRJNA1162543 | ENA

Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

Project description:Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

| PRJNA661575 | ENA

Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

Project description:Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties

| PRJNA661579 | ENA

Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties [RNA-seq]

Project description:Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties [RNA-seq]

| PRJNA661581 | ENA

Defining UHRF1 Domains That Support Maintenance of Human Colon Cancer DNA Methylation and Oncogenic Properties

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-02-28 | GSE118972 | GEO

Paneth cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-09-01 | GSE237329 | GEO

Experimentally Derived Metastasis Gene Expression Profile Predicts Recurrence and Death in Colon Cancer Patients

Project description:This SuperSeries is composed of the SubSeries listed below.

2009-11-14 | GSE17538 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data