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Transcriptional profiling of metastatic tumor cells in liver of a mouse pancreatic cancer model

ABSTRACT: Cancer patients presenting with surgically resectable disease often harbor occult metastases, a potential source of relapse that is targetable only through systemic therapy. Studies of this occult fraction have been limited by a lack of tools with which to isolate cells based on spatial grounds. We developed a photoconversion-based isolation technique allowing efficient recovery of cell clusters of any size including solitary disseminated tumor cells (DTCs), which are largely inaccessible otherwise. In a mouse pancreatic cancer model, transcriptional profiling of spontaneously arising DTCs revealed considerable heterogeneity, functionally reduced propensity to proliferate and enrichment for inflammatory-response phenotype associated with NF-κB signaling.

ORGANISM(S): Mus musculus

PROVIDER: GSE158078 | GEO | 2021/03/02

REPOSITORIES: GEO

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