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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

ABSTRACT: To study the gene expression changes upon Hoxa9 inactivation and CAF-1 suppression, we performed RNA-seq analyses at 48 hours using Hoxa9-Granulocyte-macrophage progenitor cells (Hoxa9-GMPs).

ORGANISM(S): Mus musculus

PROVIDER: GSE158227 | GEO | 2022/02/20

REPOSITORIES: GEO

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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

Project description:To study the chromatin profiles upon Hoxa9 inactivation and CAF-1 suppression, we performed ChIP seq analyses at 48 hours using Hoxa9-Granulocyte-macrophage progenitor cells (Hoxa9-GMPs).

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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

Project description:To study the expression and chromatin profiles upon Hoxa9 inactivation and CAF-1 suppression, we performed ATAC-seq and RNA-seq analyses at 48 hours using Hoxa9-Granulocyte-macrophage progenitor cells (Hoxa9-GMPs).

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CAF-1 Histone Chaperone is a Repressor of Mixed Cellular States in Myeloid Progenitors [scRNA-seq]

Project description:To characterize the cell identity of CAF-1 depleted Hoxa9-granulocyte-macrophage progenitors (Hoxa9-GMPs) and address whether CAF-1 suppression in Hoxa9-GMPs leads to either a heterogeneous or a mixed lineage state, we conducted single cell transcriptome analysis before and after the Hoxa9-GMPs commit to differentiation in both CAF-1 suppression and Hoxa9 inactivation conditions.

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Project description:Cell fate commitment is driven by dynamic changes in chromatin architecture and activity of lineage-specific transcription factors (TFs). The chromatin assembly factor-1 (CAF-1) is a histone chaperone that regulates chromatin architecture by facilitating nucleosome assembly during DNA replication. Accumulating evidence supports a substantial role of CAF-1 in cell fate maintenance, but the mechanisms by which CAF-1 restricts lineage choice remain poorly understood. Here, we investigate how CAF-1 influences chromatin dynamics and TF activity during lineage differentiation. We show that CAF-1 suppression triggers rapid differentiation of myeloid stem and progenitor cells into a mixed lineage state. We find that CAF-1 sustains lineage fidelity by controlling chromatin accessibility at specific loci, and limiting the binding of ELF1 TF at newly-accessible diverging regulatory elements. Together, our findings decipher key traits of chromatin accessibility that sustain lineage integrity and point to a powerful strategy for dissecting transcriptional circuits central to cell fate commitment.

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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

Project description:This SuperSeries is composed of the SubSeries listed below.

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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

Project description:Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

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Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

Project description:Regulation of Chromatin Accessibility by the Histone Chaperone CAF-1 Sustains Lineage Fidelity

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