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AURATUS goldfish environmental genomics projects: dopamine agonists

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Cyprinus carpio Carassius auratus

PROVIDER: GSE15855 | GEO | 2012/12/19

SECONDARY ACCESSION(S): PRJNA116955

REPOSITORIES: GEO

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AURATUS goldfish environmental genomics projects: dopamine agonists

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-12-19 | E-GEOD-15855 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: dopamine antagonists

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AURATUS goldfish environmental genomics projects: dopamine antagonists

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AURATUS goldfish environmental genomics projects: dopamine depletion in hypothalamus and telencephalon

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AURATUS goldfish environmental genomics projects: dopamine depletion in hypothalamus and telencephalon

Project description:Sexually mature (mid-May) adult female goldfish received a single injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP; 50 ug/g body weight at 5 uL/g body weight) or 0.6% saline on day 0 and a single injection of alpha-methyl-p-tyrosine (aMPT; 240 ug/g body weight at 5 uL/g body weight) or 0.6% saline on Day 5 with the goal of severely deplete catecholamine (dopamine and norepinephrine) levels. Hypothalamus tissue was extracted on Day 6. Telencephalon tissue was extracted on Day 6. The objective of this study was to examine gene expression changes in the neuroendocrine brain in response to catecholamine depletion. A common reference design was used for both the hypothalamus and telencephalon groups: Hypothalamus: 3 independent groups of hypothalami from sexually mature female goldfish treated with 50 ug/g MPTP + 240 ug/g aMPT hybridized against a common vehicle control. An additional group was used for a dye-swap. Slides 4, 17, 21, 52. Telencephalon: 3 independent groups of telencephali from sexually mature female goldfish treated with 50 ug/g MPTP + 240 ug/g aMPT hybridized against a common vehicle control. An additional group was used for a dye-swap. Slides 28, 34, 43, 48.

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AURATUS goldfish environmental genomics projects: sulpiride in the hypothalamus

Project description:Sexually regressing female goldfish were administered 2 i.p. injections: 1) 6 ug/g sulpiride in 0.1% DMSO and 0.6% saline, and; 2) 0.6% saline Tissues were harvested 5h post-injection.

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AURATUS goldfish environmental genomics projects: sulpiride in the telencephalon

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AURATUS goldfish environmental genomics projects: quinpirole in the telencephalon

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline OR quinpirole (2 ug/g) dissolved in 0.6% saline.

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AURATUS goldfish environmental genomics projects: quinpirole in the hypothalamus

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline OR quinpirole (2 ug/g) dissolved in 0.6% saline.

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AURATUS goldfish environmental genomics projects: lumiestrone in the liver

Project description:Sexually inactive male goldfish were injected i.p. with lumiestrone (0.5 µg/g) or control vehicle (in charcoal-stripped peanut oil; 5 µL/g) to investigate the biological activity of lumiestrone.

2012-06-05 | GSE12252 | GEO

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