Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Establishment of hTERT immortalized mouse cardiosphere derived cell line.

ABSTRACT: The analysis showed differences of transcriptomes between 5-azacytidine induced CDCs and control CDCs. We identified 10uM 5-azacytidine treatment for 3 days, following with normal culture for 4 weeks,could induce hTERT immortlized CDCs cardiac differentiation. Differential gene expression cluster analysis indicated big difference after 5-aza treatment, which include cardiomyocyte specific genes,such as tnnt2,desmin,mef2a,etc.

ORGANISM(S): Mus musculus

PROVIDER: GSE159640 | GEO | 2020/10/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Establishment of hTERT immortalized mouse cardiosphere derived cell line

Project description:The analysis showed differences of transcriptomes between primary CDCs and immortalized CDCs as well as revealed TGF-β inhibitor-SB431542 treatment induced expression changes. We identified hTERT immortalization could result the cell proliferation and cell cycle related genes upregulation in iCDC. KEGG results demonstrated that TGF-β inhibitor SB431542 treatment could inhibit cardiomyopathy-related pathway which indicate TGF-β signaling pathway may serve as new target for cardiomyopathy therapy, and iCDC could be used as a heart-related chemicals screening.

2019-12-31 | GSE131765 | GEO

Establishment of hTERT immortalized mouse cardiosphere derived cell line

Project description:Establishment of hTERT immortalized mouse cardiosphere derived cell line

| PRJNA544715 | ENA

Establishment of hTERT immortalized mouse cardiosphere derived cell line.

Project description:Establishment of hTERT immortalized mouse cardiosphere derived cell line.

| PRJNA669880 | ENA

Extracellular vesicles (EV) from IFNγ-primed cardiosphere-derived cells (CDCs)

Project description:Characterization of proteins in extracellular vesicles (EV) from cardiosphere-derived cells (CDCs) of a clinically relevant pig model. Additionally, considering that priming stem cells with inflammatory stimuli may increase the therapeutic potential of released vesicles, here we studied the dynamic changes that take place in the EV from IFNγ-primed CDCs.

2020-05-15 | PXD016434 | Pride

IMR90 hTERT

Project description:Transcription profiling from young and pre-senescent IMR90 cells, transfected either with hTERT (pBabe-puro-hTERT) or vector control (pBabe-puro). Population doubling values are contained in Characteristics[generation] column.

2014-06-17 | E-MTAB-2087 | biostudies-arrayexpress

Human gastric mucosa Cells: hTERT-negative para cancerous tissue (Control) vs. hTERT-positive gastric cancer tissue

Project description:The miRNA expression profiles in one pair of hTERT-positive gastric cancer tissue and an hTERT-negative para-cancerous tissue. The para-cancerous tissue is at least 5cm away from the cancer tisse. The expression of hTERT of identified by immunohistochemistry before RNA extraction for miRNA assay.

2013-05-21 | GSE47107 | GEO

Dysregulated gene expression in Mef2a knockout mice subjected to cardiotoxin-induced muscle injury

Project description:We identified genes expressed in mouse skeletal muscle, during the process of muscle regeneration after injury, which are dysregulated in the absence of Mef2a expression. MEF2A is a member of the evolutionarily conserved MEF2 transcription factor family which has known roles in cardiac muscle development and function, but is not well studied in skeletal muscle. We performed a comparison of gene expression profiles in wild type and MEF2A knockout tibialis anterior muscle, seven days post-injury with cardiotoxin. The results indicated that a variety of genes expressed during muscle regeneration, predominantly microRNAs in the Gtl2-Dio3 locus, are dysregulated by the loss of MEF2A expression. Skeletal muscle RNA used in the present study included the following two sample groups: (WT) pooled total RNA from tibialis anterior muscle taken from 5 wild type mice at seven days post-injury with 10uM cardiotoxin; (KO) pooled total RNA from tibialis anterior muscle taken from 5 Mef2a knockout mice at seven days post-injury with 10uM cardiotoxin. All mice were between 2-4 months of age. Both male and female mice were used.

2013-02-12 | E-GEOD-41871 | biostudies-arrayexpress

Human gastric mucosa: 3 paired hTERT-negative para cancerous tissue (Control) vs. hTERT-positive gastric cancer tissue

Project description:The lncRNA expression profiles in three pairs of hTERT-positive gastric cancer tissue sand hTERT-negative para-cancerous tissues. The para-cancerous tissue is at least 5cm away from the cancer tissue. The expression of hTERT of identified by immunohistochemistry before RNA extraction for lncRNA assay.

2014-06-26 | GSE58828 | GEO

Transcriptome data from U2OS cells and hTERT CRISPR clones

Project description:To assess the biological significances of p-hTERT in ALT cells, we used the CRISPR-Cas9 system targeting the hTERT gene and performed RNA-seq to analyze the profile of gene expression in hTERT-CRISPR clones (clone#1 and #2). The subsequent gene ontology (GO) analysis showed enrichment of genes involved in cell cycle process and DNA replication within genes down-regulated in hTERT-CRISPR clones.

2024-04-08 | GSE226963 | GEO

RIP-seq analysis for RNAs associated with hTERT in nuclear speckles.

Project description:To investigate the function of p-hTERT in nuclear speckles, we isolated hTERT complex by immunoprecipitation with an anti-hTERT mAb from nuclear speckles purified biochemically and conducted RIP-seq analysis for RNAs associated with hTERT in nuclear speckles.

2024-04-08 | GSE226964 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data