Transcriptomics

Dataset Information

0

Transcriptional profiling of indolent and aggressive mammary lesions


ABSTRACT: We performed transcriptional profiling of tumor cells from indolent and aggressive lesions isolated from a mouse model of breast cancer. To initiate formation of mammary lesions, we delivered lentiviral particles carrying constitutively-activated Erbb2 tagged with HA and GFP (caErbB2) through the lactiferous glands of adult virgin female. Mammary glands bearing lesions were visualized under a fluorescence stereoscope, and regions harboring aggressive (>2mm, comprised of invasive lesions) and indolent (<2mm, enriched for in situ lesions) lesions were dissected away from each other. Lesions from multiple animals were pooled to obtain sufficient material for downstream analysis. Following tissue digestion, GFP-expressing epithelial cells were isolated by fluorescence-activated cell sorting (FACS), and then subjected to bulk RNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE162005 | GEO | 2021/06/17

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2021-06-17 | GSE161983 | GEO
| PRJNA680303 | ENA
| PRJNA680306 | ENA
| PRJNA680255 | ENA
2013-01-30 | E-MIMR-17 | biostudies-arrayexpress
2010-03-04 | GSE16455 | GEO
2010-03-04 | E-GEOD-16455 | biostudies-arrayexpress
2017-01-10 | GSE84042 | GEO
2017-01-10 | GSE83917 | GEO
2014-10-18 | E-GEOD-62451 | biostudies-arrayexpress