Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pro-inflammatory monocyte phenotype during acute progression of cerebral small vessel disease

ABSTRACT: The aetiology of cerebral small vessel disease (SVD) remains elusive, though currently evidence is accumulating that inflammation contributes to its pathophysiology. In this prospective high-frequency imaging study, we showed that the incidence of acute SVD progression coincides with a functional and transcriptional pro-inflammatory monocyte phenotype. To detect SVD progression, individuals with SVD underwent monthly magnetic resonance imaging (MRI) for 10 consecutive months. RNA sequencing revealed a pro-inflammatory monocyte signature with upregulation of the EGR2 gene. Collectively, we showed that individuals with progressive SVD have pro-inflammatory circulating monocytes characterized by an augmented cytokine production capacity and a pro-inflammatory transcriptional signature.

ORGANISM(S): Homo sapiens

PROVIDER: GSE162790 | GEO | 2020/12/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Pro-inflammatory monocyte phenotype during acute progression of cerebral small vessel disease

Project description:Pro-inflammatory monocyte phenotype during acute progression of cerebral small vessel disease

| PRJNA683025 | ENA

Differential expression in the cortex and white matter of patients with cerebral small vessel disease (SVD)

Project description:In this study, we performed the first genome-wide expression profiling of post-mortem brains of a cohort of patients deceased from SVD and compared them to age-matched normal controls. Normal-appearing frontal temporal and occipital cortical and subcortical brain samples were dissected at autopsy from 5 patients diagnosed with pure SVD and 5 control patients without neurological disease and immediately frozen.

2015-03-11 | E-MTAB-3408 | biostudies-arrayexpress

Platelet-derived transcription factors license human monocyte inflammation

Project description:CD14+ monocytes, the predominant population in human blood, are primarily engaged in host defense and pro-inflammatory cytokine responses. Aberrant monocyte activity causes life-threatening cytokine storms, while dysfunctional monocytes lead to 'immunoparalysis.' Understanding the mechanisms controlling monocyte functions is therefore paramount. Here, we reveal platelets' vital role in human monocytes' pro-inflammatory responses. Natural low platelet counts in patients with immune thrombocytopenia (ITP) , platelet depletion in healthy human monocytes, or in vivo platelet depletion in mice, result in monocyte immunoparalysis, characterized by reduced pro-inflammatory gene expression and weakened cytokine responses to immune challenge. Remarkably, supplementation with fresh platelets reverses monocyte immunoparalysis. In mice, thrombocytopenia results in down-regulation of myeloid innate immune genes, and compromised host defense transcriptional programs in monocytes despite normal responses to LPS. Platelets control monocyte cytokines independently of traditional cross-talk pathways, acting as reservoirs of transcription factors like NF?B and MAPK p38. We pinpointed a vesicle-derived NF?B2 transfer to human monocytes by mass spectrometry-based proteomics. Functionally, platelets proportionally restored impaired cytokine secretion in human monocytes lacking MAPK p38a and NF?B p65 and NF?B2. We unveil the intercellular transfer of inflammatory regulators, positioning platelets as central checkpoints in monocyte-mediated inflammation.

2024-08-14 | PXD052113 | Pride

The Transcriptome Of Cmml Monocytes Is Highly Inflammatory And Reflects Leukemia-Specific And Age-Related Alterations

Project description:Chronic myelomonocytic leukemia (CMML) is an aggressive myeloid neoplasm of older individuals characterized by persistent monocytosis. Somatic mutations in CMML are heterogeneous and only partially explain the variability in clinical outcomes. Recent data suggest that cardiovascular morbidity is increased in CMML and contributes to reduced survival. Clonal hematopoiesis of indeterminate potential (CHIP), the presence of mutated blood cells in hematologically normal individuals, is a precursor of age-related myeloid neoplasms and associated with increased cardiovascular risk. To isolate CMML-specific alterations from those related to aging, we performed RNA sequencing and DNA methylation profiling on purified monocytes from CMML patients and from age-matched (old) and young healthy controls. We found that the transcriptional signature of CMML monocytes is highly pro-inflammatory, with upregulation of multiple inflammatory pathways, including tumor necrosis factor, IL-6 and IL-17 signaling, while age per se does not significantly contribute to this pattern. We observed no consistent correlations between aberrant gene expression and CpG island methylation, suggesting that pro-inflammatory signaling in CMML monocytes is governed by multiple and complex regulatory mechanisms. We propose that pro-inflammatory monocytes may contribute to cardiovascular morbidity in CMML patients, and promote progression by selection of mutated cell clones. Our data raise questions whether asymptomatic CMML patients may benefit from monocyte-depleting or anti-inflammatory therapies.

2019-10-24 | GSE135902 | GEO

Dietary intake regulates the circulating pro-inflammatory monocyte pool

Project description:Dietary intake regulates the circulating pro-inflammatory monocyte pool

| PRJNA523614 | ENA

Transcriptional signature of human pro-inflammatory TH17 cells

Project description:Transcriptional signature of human pro-inflammatory TH17 cells

| PRJNA407976 | ENA

Transcription profiling of human HCMV-Infected monocytes study 2

Project description:Human cytomegalovirus induces a pro-inflammatory monocyte following infection. To begin to address how HCMV induces these rapid changes in infected monocytes, we examined the transcriptome of infected monocytes. Global transcriptional profiling using cDNA microarrays revealed a significant number of pro-inflammatory genes were upregulated within 4 hours post infection. Experiment Overall Design: To begin to globally define the HCMV-induced changes in monocyte function, we performed a transcriptome analysis. Specifically, a cDNA microarray containing 12,626 unique probe sets was utilized to assess the modulation of the monocyte transcriptome at 4 hours post infection. A total of 6 replicates from mock-infected and 6 replicates from HCMV-infected monocytes were analyzed in this study.

2008-06-20 | E-GEOD-11408 | biostudies-arrayexpress

Rheumatoid Arthritis macrophages are primed for inflammation and display bioenergetic and functional alterations

Project description:To investigate the pro-inflammatory and metabolic function of Rheumatoid Arthritis monocyte derived macrophages RNA sequencing was perfomed on polarised healthy and Rheumatoid Arthritis monocyte-derived macrophages

2023-01-20 | GSE223325 | GEO

Expression in donor monocyte derived cells

Project description:Monocyte differentiate into macrophages that display either pro-inflammatory or tolerogenic profile depending on the inflammatory condition of the intestine. We performed adoptive monocyte transfer to dissect the dynamics of monocyte to macropahges differentiation. Gene expression analysis of donor monocyte-derived cells recovered from non-inflamed and inflamed small intestine of recipient mice at day 1, 2 and 5 after transfer for in-depth investigation of monocyte development in the intestine.

2019-06-12 | GSE132530 | GEO

Transcriptional signature of human pro-inflammatory TH17 cells [TH17 clones]

Project description:Transcriptional signature of human pro-inflammatory TH17 cells [TH17 clones]

| PRJNA408016 | ENA