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SPEN is required for Xist upregulation during initiation of X chromosome inactivation

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE163321 | GEO | 2021/09/30

REPOSITORIES: GEO

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SPEN is required for Xist upregulation during initiation of X chromosome inactivation.

Project description:At initiation of X chromosome inactivation (XCI), Xist is monoallelically upregulated from the future inactive X (Xi) chromosome, overcoming repression by its antisense transcript Tsix. Xist recruits various chromatin remodelers, amongst them SPEN, which are involved in silencing of X-linked genes in cis and establishment of the Xi. Here, we show that SPEN plays an important role in initiation of XCI. Spen null female mouse embryonic stem cells (ESCs) are defective in Xist upregulation upon differentiation. We find that Xist-mediated SPEN recruitment to the Xi chromosome happens very early in XCI, and that SPEN-mediated silencing of the Tsix promoter is required for Xist upregulation. Accordingly, failed Xist upregulation in Spen-/- ESCs can be rescued by concomitant removal of Tsix. These findings indicate that SPEN is not only required for the establishment of the Xi, but is also crucial in initiation of the XCI process.

| S-EPMC8636516 | biostudies-literature

SPEN is required for Xist upregulation during initiation of X chromosome inactivation (ChIP-seq)

Project description:At initiation of X chromosome inactivation (XCI), Xist is monoallelically upregulated from the future inactive X (Xi) chromosome, overcoming repression by its antisense transcript Tsix. Xist recruits various chromatin remodelers, amongst them SPEN, which are involved in silencing of X-linked genes in cis and establishment of the Xi. Here, we show that SPEN plays an important role in the initiation of XCI. Spen null female mouse embryonic stem cells (ESCs) are defective in Xist upregulation upon differentiation. We find that Xist-mediated SPEN recruitment to the Xi chromosome happens very early in XCI, and that SPEN-mediated silencing of the Tsix promoter is required for Xist upregulation. Accordingly, failed Xist upregulation in Spen-/- ESCs can be rescued by concomitant removal of Tsix. These findings indicate that SPEN is not only required for the establishment of the Xi, but is also crucial in the initiation of the XCI process.

2021-09-30 | GSE163319 | GEO

SPEN is required for Xist upregulation during initiation of X chromosome inactivation (RNA-seq)

Project description:At initiation of X chromosome inactivation (XCI), Xist is monoallelically upregulated from the future inactive X (Xi) chromosome, overcoming repression by its antisense transcript Tsix. Xist recruits various chromatin remodelers, amongst them SPEN, which are involved in silencing of X-linked genes in cis and establishment of the Xi. Here, we show that SPEN plays an important role in the initiation of XCI. Spen null female mouse embryonic stem cells (ESCs) are defective in Xist upregulation upon differentiation. We find that Xist-mediated SPEN recruitment to the Xi chromosome happens very early in XCI, and that SPEN-mediated silencing of the Tsix promoter is required for Xist upregulation. Accordingly, failed Xist upregulation in Spen-/- ESCs can be rescued by concomitant removal of Tsix. These findings indicate that SPEN is not only required for the establishment of the Xi, but is also crucial in the initiation of the XCI process.

2021-09-30 | GSE163320 | GEO

SPEN is required for Xist upregulation during initiation of X chromosome inactivation

Project description:SPEN is required for Xist upregulation during initiation of X chromosome inactivation

| PRJNA685753 | ENA

SPEN is required for Xist upregulation during initiation of X chromosome inactivation (ChIP-seq)

Project description:SPEN is required for Xist upregulation during initiation of X chromosome inactivation (ChIP-seq)

| PRJNA685754 | ENA

SPEN is required for Xist upregulation during initiation of X chromosome inactivation (RNA-seq)

Project description:SPEN is required for Xist upregulation during initiation of X chromosome inactivation (RNA-seq)

| PRJNA685755 | ENA

Spen regulates X chromosome inactivation

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-12-08 | GSE131784 | GEO

Xist-mediated Chromosome Inactivation

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-06-17 | GSE119607 | GEO

XIST dampens X chromosome activity in a SPEN-dependent manner during early human development

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-04-24 | GSE246643 | GEO

An Xist-activating antisense RNA required for X-chromosome inactivation.

Project description:The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.

| S-EPMC4616153 | biostudies-literature

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