Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The kinetics of gene expressions of HeLa cells treated with MT7

ABSTRACT: Uncontrolled mitosis forms basis for tumor cell proliferation. Interference with normal mitotic progression often leads tumor cells to mitotic arrest. As a consequence, cell death follows, but not at all time. Reasonably, the antimitotic therapy has been proven effective and indispensable in the clinical treatment of cancer.Recently, we generated a 6H-Pyrido[2',1':2,3]imidazo[4,5-c] isoquinolin -5(6H)-one library, several products of which had been shown to possess antimitotic activities in vitro. In an attempt to understand the antitumor mechanism of these novel chemicals, we have chosen one bioactive product of this library, MT7, as a model compound in this study We used microarrays to detail the kinetics of gene expression in HeLa cells in a time course of MT7 treatment (from 1.5 h to 12 h). The gene expression profiles were further analyzed by GSEA and Connectivity Map, respectively.

ORGANISM(S): Homo sapiens

PROVIDER: GSE16378 | GEO | 2009/06/08

SECONDARY ACCESSION(S): PRJNA115287

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of human HeLa cells treated with MT7

Project description:Uncontrolled mitosis forms basis for tumor cell proliferation. Interference with normal mitotic progression often leads tumor cells to mitotic arrest. As a consequence, cell death follows, but not at all time. Reasonably, the antimitotic therapy has been proven effective and indispensable in the clinical treatment of cancer.Recently, we generated a 6H-Pyrido[2',1':2,3]imidazo[4,5-c] isoquinolin -5(6H)-one library, several products of which had been shown to possess antimitotic activities in vitro. In an attempt to understand the antitumor mechanism of these novel chemicals, we have chosen one bioactive product of this library, MT7, as a model compound in this study; We used microarrays to detail the kinetics of gene expression in HeLa cells in a time course of MT7 treatment (from 1.5 h to 12 h). The gene expression profiles were further analyzed by GSEA and Connectivity Map, respectively. Experiment Overall Design: HeLa cells growing on the 10-cm dishes were treated with 5 mM MT7 for 0(control), 1.5(Time1), 3(Time2), 6(Time3) and 12(Time4) hours, respectively. Cells were then washed three times with PBS and the total RNA was isolated and processed for Affymetrix U133A 2.0 hybridizations.

2009-06-24 | E-GEOD-16378 | biostudies-arrayexpress

BMF repression keeps mitosis away from anoikis

Project description:FOXM1 repression increases mitotic death upon antimitotic chemotherapy through BMF upregulation

| PRJEB44770 | ENA

Effect of BRCA1 overexpression on genetic expressions of cervical cancer based on next generation sequencing

Project description:The cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (Sangon, China) and 1% antibiotic-antimitotic solution (Gibco, USA). The SiHa cells were transfected using Lipofectamine 3000 reagent following the manufacturer’s protocol and incubated for 48h for further analysis.The gene expression profiles were analyzed of SiHa cells transfected with GV230-BRCA1 or GV230 plasmid to assess effect of BRCA1 overexpression on genetic expressions of cervical cancer.

2019-12-06 | GSE141558 | GEO

Effect of FENDRR on genetic expressions of primary liver cancer based on next generation sequencing

Project description:Primary liver cancer (PLC) is the third largest contributor to cancer mortality in the world. PLC is a heterogeneous disease that encompasses several biologically distinct subtypes including hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and combined hepatocellular-cholangiocarcinoma (CHC). The cells were cultured in DMEM medium supplemented with 10% fetal bovine serum and 1% antibiotic-antimitotic solution. The HCC cell line LM3 and ICCA cell line RBE were transfected using Lipofectamine 3000 reagent following the manufacturer’s protocol and incubated for 48h for further analysis. The gene expression profiles were analyzed of LM3 and RBE cells transfected with si-FENDRR or si-NC to assess effect of FENDRR on genetic expressions of PLC.

2021-03-15 | GSE168885 | GEO

STING-dependent paracriny shapes apoptotic priming of breast tumors in response to anti-mitotic treatment

Project description:A fascinating but uncharacterized action of antimitotic chemotherapy is to collectively prime cancer cells to apoptotic mitochondrial outer membrane permeabilization (MOMP), while impacting only on cycling cell subsets. Here, we show that a proapoptotic secretory phenotype is induced by activation of cGAS/STING in cancer cells that are hit by antimitotic treatment, accumulate micronuclei and maintain mitochondrial integrity despite intrinsic apoptotic pressure. Organotypic cultures of primary human breast tumors and patient-derived xenografts sensitive to paclitaxel exhibit gene expression signatures typical of type I IFN and TNFalpha exposure. These cytokines induced by cGAS/STING activation trigger NOXA expression in neighboring cells and render them acutely sensitive to BCL-xL inhibition. cGAS/STING-dependent apoptotic effects are required for paclitaxel response in vivo, and they are amplified by sequential, but not synchronous, administration of BH3 mimetics. Thus anti-mitotic agents propagate apoptotic priming across heterogeneously sensitive cancer cells through cytosolic DNA sensing pathway-dependent extracellular signals, exploitable by delayed MOMP targeting.

2019-11-21 | GSE140489 | GEO

Effects of antimitotic drug benomyl on wild-type strain and strains with Yap1 or Pdr1 deletions

Project description:Time-course analyses of the modifications of the yeast gene expression program which immediately follows addition of the antimitotic drug benomyl. Parallel experiments were conducted in different genetic contexts using strains deleted in the Yap1 and Pdr1 genes.

2004-09-06 | E-GEOD-1617 | biostudies-arrayexpress

Kinetics of LPS induction in wt and Cop1-ko BMDMs

Project description:Bone marrow was extracted from mice that are COP1-wt Rosa26-CreERT2 or COP1-floxed Rosa26-CreERT2 BMDMs were obtained by culturing bone marrow precursors in media containing 20% of supernatant from L929 cells. At day 4 of differentiation 4-OHT was added at 1uM to induce deletion of COP1 in BMDMs derived from COP1-floxed mice. At day 7 of differentiation, BMDMs were treated with 100 ng/ml of LPS or not. BMDMs were directly harvested in lysis buffer (from Qiagen RNeasy mini kit) at different time points (0h, 2.5h, 2.5h, 4h, 6h, 9h and 13h) following LPS stimulation. Three BMDMs preparations per group: G1: BMDMs from COP1-wt mice (expressing the wt allele of COP1) CRE positive. G2: BMDMs from COP1-floxed mice (expressing the floxed allele of COP1) CRE positive

2018-10-03 | GSE114762 | GEO

Streptococcus mutans UA159

Project description:Streptococcus mutans UA159 control vs. Streptococcus mutans UA159 supplemented with 20 uM 4,5-dihydroxy-2,3-pentadione (DPD) at 3 different pH values

| PRJNA131547 | ENA

Differential Expressions of miRNAs in resting and activated human in vitro-differentiated NK cells

Project description:To investigate miRNA expressions of miRNA upon activation. In vitro-differentiated human NK cells were freshly incubated in the presence of IL15 after 24h deprivation of IL-15. The cells were harvested at the times (0h, resting-Sample 1 and 2; 6h, activated-Sample 3 and 4) and analyzed by microarray.

2011-11-21 | E-GEOD-32411 | biostudies-arrayexpress

Kinetics of the Arabidopsis organellar and nuclear (post)transcriptomes under drought stress

Project description:We extended the investigation of nuclear transcript changes under drought to an overarching characterization of the post(transcriptome), including alternative splicing of nuclear transcripts, accumulation of organellar (chloroplast and mitochondrion) transcripts, editing and splicing of organellar transcripts, and furthermore, accumulation of transposable elements. To this end, long non-coding (lncRNA-Seq) instead of mRNA-Seq was applied, because the lncRNA-Seq workflow employs library preparation after ribosomal RNA depletion instead of library preparation of enriched mRNAs (mRNA-Seq).

2023-08-09 | GSE202931 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data