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RNA expression in ventilated preterm lamb lungs [RNA-seq]

ABSTRACT: Purpose: The goal of this study was to measure by RNA-seq the impact of preterm birth and invasive mechanical ventilation verses noninvasive respiratory support on the lung transcriptome Methods: Lung mRNA profiles were generated for: 1) Unventilated preterm lamb, 2) preterm lambs delivered at gd131 and intubated and mechanically ventilated for 3 days, 3) preterm lambs delivered at gd131 and not intubated and resuscitated by placing a face mask over the nose and mouth and controlling O2 delivery via a computer-controlled electronic blower device, 4) unventilated naturally delivered full-term lambs. Results: Using an optimized data analysis workflow, we mapped between 61 and 81 million sequence reads per sample to the sheep genome Oar_v3.1/oviAri3 Conclusions: Our study represents the first detailed analysis of ventilated preterm lung transcriptomes, with biologic replicates, generated by RNA-seq

ORGANISM(S): Ovis aries

PROVIDER: GSE163806 | GEO | 2021/06/30

REPOSITORIES: GEO

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Genome-wide DNA access sites in ventilated preterm lamb lungs [ATAC-seq]

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RNA expression in ventilated preterm lamb lungs [RNA-seq]

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Transcriptional Profiling of Lung Macrophages from Preterm Infants Identifies Disease Related Programs

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