Proteomics

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Proteomics analysis of preterm lung tissue with mass spectrometry to understand the impact of tidal volume strategy at birth

ABSTRACT: This study aimed to use a lamb model of preterm birth to investigate the impact of different tidal volume strategies, applied during positive pressure ventilation, on lung injury development. Preterm lambs were ventilated for a total of 15 minutes, followed by a 30 minute ‘rest’ period where lambs were supported via the intact placenta. A group of lambs was also euthanised at birth to act as an unventilated control group. At post-mortem lung tissue was sampled for proteomic analysis. Lung tissue samples were analysed by mass spectrometry-based proteomics using a TMT-11plex labelling approach, followed by nano-liquid chromatography coupled to an Q Exactive HF-X benchtop Orbitrap mass spectrometer in a data-dependent acquisition mode.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Ovis Aries

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Mixed Disorder As Reaction To Stress

SUBMITTER: david greening  

LAB HEAD: David Greening & Prudence Pereira-Fantini

PROVIDER: PXD041917 | Pride | 2023-10-09

REPOSITORIES: Pride

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Publications

Impact of tidal volume strategy at birth on initiating lung injury in preterm lambs.

Pereira-Fantini Prue M PM   Kenna Kelly R KR   Fatmous Monique M   Sett Arun A   Douglas Ellen E   Dahm Sophia S   Sourial Magdy M   Fang Haoyun H   Greening David W DW   Tingay David G DG  

American journal of physiology. Lung cellular and molecular physiology 20230920 5

Tidal ventilation is essential in supporting the transition to air-breathing at birth, but excessive tidal volume (V<sub>T</sub>) is an important factor in preterm lung injury. Few studies have assessed the impact of specific V<sub>T</sub> levels on injury development. Here, we used a lamb model of preterm birth to investigate the role of different levels of V<sub>T</sub> during positive pressure ventilation (PPV) in promoting aeration and initiating early lung injury pathways. V<sub>T</sub> was  ...[more]

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