MiRNA sequencing of circCpsf6 silencing models in N2A cells
Ontology highlight
ABSTRACT: To search and identify the potential downstream miRNAs of circCpsf6, miRNA sequencing analysis was performed on circCpsf6 silencing models in N2A cells. The differential expression of miRNAs caused by the knockdown of circCpsf6 was revealed in the volcano plot.Heatmaps indicated the upregulated and downregulated clusters of differential miRNAs in N2A cells when circCpsf6 was silenced.
Project description:mRNA sequencing was performed to explore involved mRNAs when circCpsf6 was silenced in N2A cells.The differential expression of mRNAs caused by the knockdown of circCpsf6 was revealed in the volcano plot.Heatmaps indicated the upregulated and downregulated clusters of differential mRNAs when circCpsf6 was silenced in N2A cells
Project description:APP misexpression plays a crucial role in triggering a complex pathological cascade, leading to Alzheimer’s disease (AD). The aim of this study is for determine the influence of APP ectopic expression on the miRNA profiles of neuronal exosomes. In study, miRNA sequencing was done using the exosomes derived from N2A (control) and APP-N2A (N2A with APP overexpression).
Project description:We compared the gene expression in untransfected N2A cells and in pCDNA3.1myc/his vector transfected N2A cells 1 sample each of untransfected and transfected N2A cells were analyzed
Project description:To analyze the role of Fus, Ewsr1, and Taf15 in alternative RNA processing, we performed exon array analysis in N2A cells using exon arrays. N2A cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions.