Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression and alternative splicing profiles of N2a siEwsr1 cells

ABSTRACT: N2a cells treated with either control or siEwsr1 oligonucleotides

ORGANISM(S): Mus musculus

PROVIDER: GSE173439 | GEO | 2022/04/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression and alternative splicing profiles of N2a siEwsr1 cells

Project description:Gene expression and alternative splicing profiles of N2a siEwsr1 cells

| PRJNA725528 | ENA

miRNA sequencing of exosomes derived from N2A and APP-N2A cells

Project description:APP misexpression plays a crucial role in triggering a complex pathological cascade, leading to Alzheimer’s disease (AD). The aim of this study is for determine the influence of APP ectopic expression on the miRNA profiles of neuronal exosomes. In study, miRNA sequencing was done using the exosomes derived from N2A (control) and APP-N2A (N2A with APP overexpression).

2022-03-30 | E-MTAB-11106 | biostudies-arrayexpress

Transcriptome anaylisis of WT and GFAT-1 G451E engineered N2a cells by RNA-Seq

Project description:Transcriptome of N2a cells carrying a GFAT-1 gain-of-function point mutation that results in a G451E amino acid substitution were compared to WT N2a transcriptome profiles. Three replicates of both cell lines were analyzed. Additionally WT N2a cells were treated with 10 mM GlcNAc for 24h prior to transcriptome analysis resulting in a third dataset with 3 replicates.

2019-11-14 | GSE140357 | GEO

Gene Expression and Exon Splicing Change Analysis of Mouse N2A Cell Transcriptome upon Polypyrimidine tract-binding protein depletion.

Project description:Purpose : Splicing factors regulate splice site choices in pre-mRNA and determine final exon set in mRNA. To understand mechanisms of splicing regulation, it is important to identify and characterize exon targets of splicing factors. Recently, development of RNA-seq technology enables researchers to investigate exon splicing profiles as well as gene expression profiles in transcriptome-wide. The goal of this study is to investigate transcriptome changes by splicing factors, Polypyrimidine Tract Binding proteins (PTB). In this study, we analyzed exon and gene expression changes followed by Ptbp1 knock down. Methods : The knockdown experiment was performed in mouse neuroblastoma (N2A) cells. Total RNA was collected from cells and further treated with DNase I to avoid DNA contamination. RNA-seq libraries were constructed in a strand specific way using dUTP and Uracil-Specific Excision Reagent enzyme. The libraries were subjected to 100bp paired-end sequencing (Illumina HiSeq2000 platform). Poly(A)-mRNA and exon profiles of N2A mouse blastoma cells in two samples: shRNA transfection control, single knock down of ptbp1. RNA-seq libraries were generated in strand specific way using dUTP and USER enzyme and sequenced using Illumina HiSeq2000.

2013-12-01 | E-GEOD-45119 | biostudies-arrayexpress

miRNA sequencing of exosomes derived from N2A and APP-N2A cells

Project description:miRNA sequencing of exosomes derived from N2A and APP-N2A cells

| PRJEB48466 | ENA

Evolution of an lncRNA leads to a primate specific modulation of alternative splicing

Project description:We performed the poly(A)-seq of 293T,Hela,THP-1 and N2a cell treated with si5S-OT,shPTBP1 or siU2AF65

2016-10-01 | GSE85709 | GEO

Comparing gene expression in untransfected and transfected mouse neuronal (N2A) cell line

Project description:We compared the gene expression in untransfected N2A cells and in pCDNA3.1myc/his vector transfected N2A cells 1 sample each of untransfected and transfected N2A cells were analyzed

2012-02-29 | E-GEOD-36142 | biostudies-arrayexpress

mRNA sequencing of circCpsf6 silencing models in N2A cells

Project description:mRNA sequencing was performed to explore involved mRNAs when circCpsf6 was silenced in N2A cells.The differential expression of mRNAs caused by the knockdown of circCpsf6 was revealed in the volcano plot.Heatmaps indicated the upregulated and downregulated clusters of differential mRNAs when circCpsf6 was silenced in N2A cells

2021-08-01 | GSE164028 | GEO

Results of RNA-Seq in Mn-treated N2a cells

Project description:Results of RNA-Seq in Mn-treated N2a cells

| PRJNA781147 | ENA

Exon array analysis of N2A cells after knocking down FUS

Project description:To analyze the role of Fus, Ewsr1, and Taf15 in alternative RNA processing, we performed exon array analysis in N2A cells using exon arrays. N2A cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions.

2020-08-10 | GSE60694 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data