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Targeted transposon screening in bacteria by CRISPR/Cas12k-guided transposase

ABSTRACT: Here, we report a CRISPR/Cas12k-transposon-assisted genome engineering (CTAGE) method that allows for high-throughput site-specific mutagenesis in microbial genomes. Exploiting the powerful CTAGE technique, we construct a site-specific transposon mutant library focusing on all the possible transcription factors (TFs) in Pseudomonas aeruginosa, enabling comprehensive identification of essential genes and new factors for antibiotic resistance.

ORGANISM(S): Pseudomonas aeruginosa PAO1

PROVIDER: GSE164797 | GEO | 2021/08/10

REPOSITORIES: GEO

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