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Antigen calibration of T Cell Receptor signalling strength and T cell differentiation in vivo

ABSTRACT: Here we perform QuantSeq 3' mRNA sequencing of RNA extracted from flow sorted splenic T cell from Tg4 Nr4a3-Tocky Tiger mice immunised with a high or low dose of MBP 4Y peptide, enabling identification of time and dose dependent transcriptional signatures

ORGANISM(S): Mus musculus

PROVIDER: GSE165817 | GEO | 2021/07/30

REPOSITORIES: GEO

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Qualitative changes in activated T cells induced by checkpoint blockade in vivo

Project description:Here we perform QuantSeq 3' mRNA sequencing of RNA extracted from flow sorted splenic T cell from Tg4 Nr4a3-Tocky Tiger mice that had been immunised with 80ug of 4Y MBP peptide for 24 hours. After 24 hours mice were re-stimulated with 8ug 4Y MBP in the presence of isotype, anti-PD1 or anti-Lag3 antibodies for 4 hours. The results show that anti-PD1 but not anti-Lag3 imparts a unique transcriptional signature on responding T cells, indicative of stronger TCR signalling.

2021-07-30 | GSE165818 | GEO

Comparison of IL10-eGFP+ cells with IL10-eGFP- CD4+ T cells induced by in vivo immunisation

Project description:Here we perform QuantSeq 3' mRNA sequencing of RNA extracted from flow sorted splenic CD4+ Il10-eGFP+ or CD4+Il10eGFP- T cells from Tg4 Nr4a3-Tocky Il10-eGFP mice 24 hours after immunisation with 4 mg/kg of [4Y]-MBP. Our aim was to confirm the Tr1 cell phenotype within the Il10-eGFP+ T cell subset

2024-12-09 | GSE283286 | GEO

Escalating-dose peptide immunotherapy induces progressive immunoregulatory changes in self-reactive T cells

Project description:Peptide immunotherapy aims to specifically restore tolerance to the administered self-antigen and prevent autoimmunity without the perturbation of normal immune function. We have developed a dose escalation protocol for subcutaneous delivery of the MHC II-restricted myelin basic protein peptide analogue Ac1-9[4Y] to T cell receptor transgenic (Tg4) mice. Dose escalation allows safe administration of high doses of peptide, which effectively induces antigen-specific tolerance and suppresses the development of experimental autoimmune encephalomyelitis, a model for the human condition multiple sclerosis. CD4+ T cells isolated from treated mice are anergic and suppressive in vitro and respond to stimulation by secretion of the immunoregulatory cytokine IL-10. To understand the molecular changes occurring throughout the course of dose-escalation immunotherapy, we undertook microarray analysis of CD4+ T cells at different the stages of treatment, using Tg4 Rag-1 deficient mice, which lack naturally occurring regulatory T cells and have a monoclonal CD4+ T cell population

2014-07-14 | GSE46036 | GEO

Transcription profiling of mouse MBP Ac 11-11 activated purified CD4+ T cells

Project description:Local catabolism of the amino acid tryptophan (Trp) by indoleamine-2,3-dioxygenase (IDO) is considered an important mechanism of regulating T cell immunity. We show that IDO transcription was increased upon stimulation of myelin-specific T cells with tolerogenic altered self-peptides. Catabolites of Trp suppressed proliferation of myelin-specific T cells and inhibited production of proinflammatory TH1 cytokines. N-(3,4,-dimethoxycinnamoyl) anthranilic acid (3,4-DAA), an orally active synthetic derivative of the Trp metabolite anthranilic acid, reversed paralysis in mice with experimental autoimmune encephalomyelitis, a model of multiple sclerosis (MS). Trp catabolites and their derivatives offer a new strategy for treating TH1-mediated autoimmune diseases, such as MS. 4Y vd 1-11 comparison files are available as supplementary files. Experiment Overall Design: Double stranded cDNA was generated from total RNA extracted from MBP Ac 11-11 activated purified CD4+ T cells. Biotinylated cRNA was manufactured by in vitro transcription and hybridized to the Affymetrix genechip murine Mu11k set according to the manufacturer's protocols. Results were calculated as the ratio of mean average differences for each gene in 4Y versus Ac1-11 stimulated samples.

2008-06-12 | E-GEOD-3401 | biostudies-arrayexpress

Transcription profiling of chicken primary bronchus and secondary bronchi on birds vaccinated under various adjuvants and challenged by avian influenza virus H9N2.

Project description:Sylvia Reemers: chicken in vivo infection: timecourse, groupI= non immunised non challenged, groupIII= non immunised challenged, groupIV= immunised challenged, groupV= immunised+w/o challenged, groupVII= immunised+Al(OH)3 challenged, groupIX= immunised+CpG challenged Tissue type= Lung1. The aim of this study was to get a better understanding of the characteristics of a protective response and provide a signature of protection after vaccination with H9N2 adjuvanted with different immunomodulators.

2010-04-11 | E-MTAB-136 | biostudies-arrayexpress

Transcription profiling of chicken paleopulmonic parabronchi on birds vaccinated under various adjuvants and challenged by avian influenza virus H9N2.

Project description:Sylvia Reemers: chicken in vivo infection: timecourse, groupI= non immunised non challenged, groupIII= non immunised challenged, groupIV= immunised challenged, groupV= immunised+w/o challenged, groupVII= immunised+Al(OH)3 challenged, groupIX= immunised+CpG challenged Tissue type= Lung4. The aim of this study was to get a better understanding of the characteristics of a protective response and provide a signature of protection after vaccination with H9N2 adjuvanted with different immunomodulators.

2010-04-01 | E-MTAB-137 | biostudies-arrayexpress

Transcription profiling of chicken trachea on birds vaccinated under various adjuvants and challenged by avian influenza virus H9N2.

Project description:Sylvia Reemers: chicken in vivo infection: timecourse, groupI= non immunised non challenged, groupIII= non immunised challenged, groupIV= immunised challenged, groupV= immunised+w/o challenged, groupVII= immunised+Al(OH)3 challenged, groupIX= immunised+CpG challenged Tissue type= Upper trachea. The aim of this study was to get a better understanding of the characteristics of a protective response and provide a signature of protection after vaccination with H9N2 adjuvanted with different immunomodulators.

2010-04-01 | E-MTAB-138 | biostudies-arrayexpress

Postnatal glucocorticoids suppress myelination in a dose-dependent manner by genomic mechanism

Project description:Objective: Postnatal glucocorticoids (GCs) are widely used in the prevention of chronic lung disease in premature infants. However, their use is associated with neurodevelopmental delay and cerebral palsy. We hypothesized that postnatal dexamethasone or betamethasone in high-dose, but not low-dose, would induce hypomyelination, astrogliosis, and motor impairment in premature rabbit pups. Additionally, these effects would be mediated by glucocorticoid receptors (GRs). Methods: Preterm rabbit pups, delivered by C-section at E29 (term=32d), were treated with a high dose of dexamethasone or vehicle. Myelin basic protein (MBP), glial fibrillary basic protein (GFAP), oligodendrocyte proliferation and maturation, and alteration of transcriptomic profile were evaluated in these pups. Neurobehavioral assessments were performed at 14d. Results: High-dose dexamethasone treatment reduced MBP expression and induced motor-impairment compared with controls. High-dose dexamethasone induced astrogliosis and altered genes associated with myelination, cell-cycle, GR, and MAP-Kinase signaling. Interpretation: High-dose postnatal dexamethasone arrested maturation of oligodendrocytes, and induced hypomyelination, gliosis and motor deficits. GC treatment reduced myelination by genomic GR-dependent mechanisms, and caused astrogliosis by non-genomic mechanisms. Two-condition experiment: forebrains of HDD (high dose dexamethasone) vs. CTR (PBS) post-natally expossed rabbit pups. Biological replicates: 4 CTR replicates, 4 HDD replicates.

2013-02-27 | E-GEOD-44610 | biostudies-arrayexpress

Postnatal glucocorticoids suppress myelination in a dose-dependent manner by genomic mechanism

2013-02-27 | GSE44610 | GEO

Expression data from 9-day-old rat Sertoli cells and MBP-treated rat Sertoli cells

Project description:Low-dose MBP can stimulated 9-day-old Sertoli cells proliferation and activated proliferation related signal pathway. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated and down-regulated genes during this process.

2016-06-09 | GSE83106 | GEO

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