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Endothelial progenitor migration and differentiation is regulated by distinct transcriptional effectors of Cloche/Npas4l

ABSTRACT: Embryonic cells including endothelial progenitors undergo extensive migration and differentiation events; however, whether and how these processes are interrelated remains unclear. The transcription factor Npas4l is necessary for endothelial specification in zebrafish by inducing the expression of the transcription factor genes etsrp, tal1 and lmo2. We generated a knock-in reporter in the npas4l locus to visualize endothelial progenitors and their derivatives in wild-type and mutant embryos. We find that in npas4l mutants, npas4l reporter expressing cells do not migrate to the midline and instead contribute to skeletal muscle and pronephric tubules. Investigating the Npas4l transcriptional effectors, we find that npas4l reporter expressing cells in tal1 mutants fail to migrate while those in etsrp mutants migrate but fail to differentiate. In lmo2 mutants, npas4l reporter expressing cells migrate and differentiate, but many express pronephric tubule markers. Altogether, these data reveal the complex regulation of endothelial progenitor migration, differentiation and fate restriction.

ORGANISM(S): Danio rerio

PROVIDER: GSE166396 | GEO | 2022/07/11

REPOSITORIES: GEO

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