Transcriptomics

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RNA-sequencing analysis of effect of miR-155 overexpression in human CD14+ monocytes


ABSTRACT: We report the application of bulk RNA-Sequencing to assess the effect of miR-155 overexpression by profiling gene expression following transfection of miR-155 mimic compared against negative control mimic in human peripheral blood CD14+ monocytes. Isolated human CD14+ monocytes were transfected for 24 hours and mRNA profiles of miR-155 and negative control mimic were generated by bulk RNA-Sequencing in 5 donors. The sequence reads passed quality checks and were analysed using DeSeq2. We mapped the sequence reads to the human genome (build hg38) and identified 16,014 transcripts.

ORGANISM(S): Homo sapiens

PROVIDER: GSE166605 | GEO | 2022/02/07

REPOSITORIES: GEO

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