Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis of ASCL1-induced neuronal differentiation in WT and MED25 knockout Glioblastoma Stem Cells

ABSTRACT: Glioblastoma stem cells were infected with lentivirus expressing a control (gRNA-AAVS1) or MED25 targeting gRNA to assess the role of MED25 in the stem cell compartment of Glioblastoma stem cells. Cells were the subjected to doxycycline-induced ASCL1 overexpression (+DOX) to induced neuronal differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE166979 | GEO | 2023/06/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq analysis of ASCL1-induced neuronal differentiation in WT and MED25 knockout Glioblastoma Stem Cells

Project description:RNA-seq analysis of ASCL1-induced neuronal differentiation in WT and MED25 knockout Glioblastoma Stem Cells

| PRJNA702509 | ENA

ChIP-seq analysis for H3K27ac in GI-MEN DOX-ASCL1 cells and for ASCL1-HA in GI-MEN DOX-ASCL1-tag-HA cells.

Project description:ChIP-seq analysis was performed in an neuroblastoma cell line to analyze DNA bindings of H3K27ac in GI-MEN DOX-ASCL1 cells and ASCL1-HA in GI-MEN DOX-ASCL1-tag-HA cells.

2023-11-21 | GSE214791 | GEO

RNA-sequencing WT vs SOCS3 knockout Glioblastoma stem-cells

Project description:Glioblastoma stem cells were infected with lentivirus expressing a non-targeting (gRNA-LacZ) or SOCS3 targeting gRNA to assess the function of SOCS3 in the stem cell compartment of Glioblastoma

2019-03-01 | GSE122486 | GEO

H3K27ac HiChIP analysis in GI-MEN DOX-ASCL1 cells

Project description:H3K27ac HiChIP analysis was performed in GI-MEN DOX-ASCL1 cells to analyze active chromatin-chromatin interactions in GI-MEN DOX-ASCL1 cells.

2023-11-21 | GSE214792 | GEO

shRNA-mediated knockdown of ETV4 and MED25 in the prostate cell line PC3 reveals set of genes potentially coregulated by MED25 and ETV4

Project description:Our in vitro binding studies support a model whereby MED25 exhibits multivalent interactions with a subset of related ETS factors, ETV1/4/5. We hypothesize that the interaction would allow for coregulation of genes by ETV1/4/5 and MED25, acting perhaps to link the ETVs to the Mediator complex. To explore this possibility, we compared the genome occupancy for FLAG-tagged MED25 and ETV4 in the prostate cancer cell line PC3, which overexpresses ETV4. We also tested for relevance of MED25 and ETV4 binding to for gene expression in PC3s. We found a high degree of overlap in the FLAG-MED25 and ETV4 ChIPs datasets consistent with our model, and also identified a subset of target genes co-dependent on Med25 and ETV4.

2019-06-28 | GSE133447 | GEO

Genome-wide occupancy of FLAG-MED25 and ETV4 in PC3 cells

2019-06-28 | GSE133445 | GEO

ATAC-seq analysis for GI-MEN DOX-ASCL1 cells and GI-MEN DOX-ASCL1-4TFs cells.

Project description:ATAC-seq analysis for GI-MEN DOX-ASCL1 cells and GI-MEN DOX-ASCL1-4TFs cells.

| PRJNA887092 | ENA

ChIP-seq analysis for H3K27ac in GI-MEN DOX-ASCL1 cells and for ASCL1-HA in GI-MEN DOX-ASCL1-tag-HA cells.

Project description:ChIP-seq analysis for H3K27ac in GI-MEN DOX-ASCL1 cells and for ASCL1-HA in GI-MEN DOX-ASCL1-tag-HA cells.

| PRJNA887088 | ENA

ChIP NS5 Ascl1-ERT2

Project description:Here we performed a ChIP-seq experiment on a sample of adherent cultures of mouse neural stem cells (NS5 cell line) under normal growth conditions and upon short term activation (30 minutes) of an inducible version of the proneural factor Mash1/Ascl1 (Ascl1-ERT2). This resulted in the generation of a genome-wide map of Ascl1 binding to chromatin.

2015-02-16 | E-MTAB-2384 | biostudies-arrayexpress

Silencing of ASCL1 in U87MG glioma cells

Project description:ASCL1 is known to act as transcriptional activator of notch signaling pathway. We have found that ASCL1 is over expressed in secondary glioblastoma. In order to delineate its functional role in gliomagensis we have used gene silencing approach to identify genes differentially regulated upon ASCL1 down regulation in U87MG glioma cell line.

2017-05-22 | GSE76652 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data