Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNAseq of CD45 enriched cells from spinal cords of Optn floxed and KO mice

ABSTRACT: We prepared spinal cords from Optn floxed and KO mice treated with vehicle or the RIPK1 inhibitor Nec-1s, which were enriched for CD45+ cells and single cell RNA sequenced using the inDrop protocol.

ORGANISM(S): Mus musculus

PROVIDER: GSE167327 | GEO | 2021/03/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

scRNAseq from spinal cords of SOD1-G93A and WT mice

Project description:We prepared spinal cords from SOD1-G93A and WT mice treated with vehicle or the RIPK1 inhibitor Nec-1s, which were single cell RNA sequenced using the DropSeq protocol.

2021-03-24 | GSE167198 | GEO

scRNAseq of CD45 enriched cells from spinal cords of Optn floxed and KO mice

Project description:scRNAseq of CD45 enriched cells from spinal cords of Optn floxed and KO mice

| PRJNA704318 | ENA

scRNAseq of FACS-isolated microglia from spinal cords of Optn KO mice

Project description:CD11b+ CX3CR1+ CD45-Int microglia were FACSorted from Optn KO mice and single cell RNA sequenced using the SmartSeq2 protocol.

2021-03-24 | GSE167331 | GEO

scRNAseq of FACS-isolated microglia from spinal cords of Optn KO mice

Project description:scRNAseq of FACS-isolated microglia from spinal cords of Optn KO mice

| PRJNA704326 | ENA

RIPK1 kinase-dependent gene expression in human astrocytes in vitro

Project description:To determine the role of RIPK1 kinase-dependent transcriptional signaling in human fetal-derived astrocytes, we stimulated cells with the RIPK1 kinase-activating stimulus TNF/Smac/zVAD in the presence or absence of the RIPK1 kinase inhibitor Nec-1s. We identified various genes modulated in a RIPK1 kinase-dependent manner in human astrocytes, and the main biological pathways were related to an interferon signaling and anti-viral response.

2021-08-12 | GSE172027 | GEO

RIPK1 kinase-dependent gene expression in mouse microglia and astrocytes in vitro

Project description:To determine the role of RIPK1 kinase-dependent transcriptional signaling in microglia and astrocytes, we stimulated cells with RIPK1 kinase-activating stimuli TNF/5z-7 and TNF/Smac/zVAD in the presence or absence of the RIPK1 kinase inhibitor Nec-1s. We identified various genes modulated in a RIPK1 kinase-dependent manner with each stimulation in both microglia and astrocytes, and the main biological pathways were related to an inflammatory and immune response. We identified many cytokines and chemokines upregulated in both microglia and astrocytes upon RIPK1 kinase activation, demonstrating the contribution of RIPK1 kinase signaling to pro-inflammatory responses in microglia and astrocytes

2021-08-12 | GSE154230 | GEO

The OPTN interactome using BioID

Project description:We use a proximity labelling-based proteomics strategy (BioID) to map the interactome of OPTN. OPTN was tagged N and C-terminally with BirA* in RPE1 cells and then, after labelling with biotin, streptavidin pull downs were performed to identify OPTN-proximal proteins.

2020-06-16 | PXD019386 | Pride

Genome wide Affymetric Microarray analysis for RIPK1 dependent inflammatory changes in Bone Marrow Derived Macrophages (BMDMs)

Project description:Gene level expression estimate using the Whole Transcript (WT) Assay approach of the Gene 1.0 ST Array System for Mouse. This assay was done to identify the RIPK1-dependent gene expression changes in mouse BMDMs. Cost-effective gene-level analysis based on whole-transcript coverage. We analyzed Bone Marrow Derived Macrophages (BMDMs) under 4 different conditions (Control, LPS, LPS/zVAD, LPS/zVAD/Nec-1) to assess inflammatory changes in RIPK1 kinase dependent manner compared to LPS, LPS/zVAD plus RIPK1 inhibitor Nec-1 and control.

2016-09-07 | E-GEOD-72797 | biostudies-arrayexpress

Discovery of a cooperative mode of inhibiting RIPK1 kinase

Project description:By using Limited Proteolysis-Mass Spectrometry (LiP-MS) and Hydrogen Deuterium Exchange-Mass Spectrometry (HDX-MS) analysis, we identified the binding pocket of Nec-34 in RIPK1 kinase domain. This newly identified binding pocket of Nec-34 is distinct from distinct from that of Nec-1s.

2021-03-07 | PXD024560 |

OPTN as a therapeutic target for acetaminophen-induced liver injury by activating mitophagy

Project description:Acetaminophen (APAP) is a leading cause of acute liver failure in Western countries result from the accumulation of damaged mitochondria. However, there is no related clinically useful therapeutic intervention. Optineurin (OPTN) is a multifunctional protein involved in autophagy, oxidative stress, as well as nuclear factor κB (NF-κB) and IRF3 signaling, and OPTN mutations are associated with several human diseases.Here, to study the role of OPTN in APAP induced liver injury, we performed RNA-seq analysis of isolated control and Optn-/- mouse liver tissues.

2024-08-21 | GSE195753 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data