Proteomics

Dataset Information

0

The OPTN interactome using BioID


ABSTRACT: We use a proximity labelling-based proteomics strategy (BioID) to map the interactome of OPTN. OPTN was tagged N and C-terminally with BirA* in RPE1 cells and then, after labelling with biotin, streptavidin pull downs were performed to identify OPTN-proximal proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Htert-rpe1 Cell, Cell Culture

SUBMITTER: Thomas O'Loughlin  

LAB HEAD: Folma Buss

PROVIDER: PXD019386 | Pride | 2020-06-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BOPTN_54735.raw Raw
BOPTN_54736.raw Raw
BOPTN_54737.raw Raw
BirA_190517_070617.raw Raw
BirA_280517_070617.raw Raw
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Publications

OPTN recruitment to a Golgi-proximal compartment regulates immune signalling and cytokine secretion.

O'Loughlin Thomas T   Kruppa Antonina J AJ   Ribeiro Andre L R ALR   Edgar James R JR   Ghannam Abdulaziz A   Smith Andrew M AM   Buss Folma F  

Journal of cell science 20200615 12


Optineurin (OPTN) is a multifunctional protein involved in autophagy and secretion, as well as nuclear factor κB (NF-κB) and IRF3 signalling, and <i>OPTN</i> mutations are associated with several human diseases. Here, we show that, in response to viral RNA, OPTN translocates to foci in the perinuclear region, where it negatively regulates NF-κB and IRF3 signalling pathways and downstream pro-inflammatory cytokine secretion. These OPTN foci consist of a tight cluster of small membrane vesicles, w  ...[more]

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