Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression response of Populus tremuloides cell suspension cultures to methyl jasmonate feeding

ABSTRACT: Gene expression response of Populus cell cultures subjected to methyl jasmonate feeding was analyzed using the Affymetrix poplar genome microarrays. Keywords: Stress response

ORGANISM(S): Populus sp. Populus tremuloides

PROVIDER: GSE16773 | GEO | 2009/12/01

SECONDARY ACCESSION(S): PRJNA122651

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of Populus tremuloides cell suspension cultures to methyl jasmonate feeding

Project description:Gene expression response of Populus cell cultures subjected to methyl jasmonate feeding was analyzed using the Affymetrix poplar genome microarrays. Experiment Overall Design: Populus tremuloides suspension cells wereÂ treated with methyl jasmonate (MJ) in DMSO orÂ with DMSO alone (C).Â Cells were harvested after 48 hours.Â Two biological replicates were obtained for each condition.

2009-12-14 | E-GEOD-16773 | biostudies-arrayexpress

Response of aspen cell cultures to combinatorial feeding of methyl jasmonate and AOPP.

Project description:To better understand the role of phenylpropanoid pathway perturbation in plant metabolism, Populus tremuloides cell cultures previously established from leaf mesophyll tissue were fed with methyl jasmonate, alpha-aminooxy-beta-phenylpropionic acid (AOPP), or both approximately 5 days after transfer to fresh medium. Methyl jasmonate is an elicitor that activates a suite of defense responses including phenylpropanoid metabolism. In contrast, AOPP acts as an inhibitor of phenylalanine ammonia lyase (PAL). Samples were harvested 48 hrs after initiation of the experiment. Total RNA was extracted and gene expression measured using Affymetrix poplar genome microarrays.

2022-05-06 | GSE154224 | GEO

Response of aspen cell cultures fed piperonylic acid to methyl jasmonate.

Project description:To better understand the role of phenylpropanoid pathway perturbation in plant metabolism, Populus tremuloides cell cultures previously established from leaf mesophyll tissue were fed with piperonylic acid (PIP) alone, or in combination with methyl jasmonate (MJ_PIP) approximately 5 days after transfer to fresh medium. Methyl jasmonate is an elicitor that activates a suite of defense responses including phenylpropanoid metabolism, while PIP acts as an inhibitor of cinnamate-4-hydroxylase (C4H). Sample tissues were contemporary with those used to generate Series GSE16773. Samples were harvested 48 hrs after initiation of the experiment. Total RNA was extracted and gene expression measured using Affymetrix poplar genome microarrays.

2022-05-06 | GSE154225 | GEO

Transcription profiling of Populus stress response: nitrogen limitation, leaf wounding, and methyl jasmonate feeding of cell culture

Project description:This SuperSeries is composed of the following subset Series:; GSE16773: Gene expression response of Populus tremuloides cell suspension cultures to methyl jasmonate feeding; GSE16783: Wound-induced gene expression changes in Populus: 1 week; GSE16785: Wound-induced gene expression changes in Populus: 90 hours; GSE14893: Comparative transcriptomics analysis of Populus leaves under nitrogen limitation: clone 3200; GSE14515: Comparative transcriptomics analysis of Populus leaves under nitrogen limitation: clone 1979 Experiment Overall Design: Refer to individual Series

2009-12-14 | E-GEOD-16786 | biostudies-arrayexpress

Effect of Methyl jasmonate of the Poplar root transcriptome

2014-04-17 | GSE56864 | GEO

Effect of Methyl jasmonate of the Poplar root transcriptome

Project description:Illumina HiSeq technology was used to generate mRNA profiles from Populus tremula x alba INRA 717-1B4 roots treated with Methyl jasmonate. Samples were harvested after two weeks either from untreated control roots or from Methyl jasmonate treated roots. Paired-end (2X100bp) reads were generated and aligned to the Populus trichocarpa (http://www.phytozome.net/poplar.php) using CLC Genomics Workbench 6. mRNA profiles from Populus tremula x alba INRA 717-1B4 roots treated with Methyl jasmonate as well as from control roots were generated by paired-end (2X100bp) Illumina HiSeq sequencing. Four samples were sequenced per lane, two biological replicates per treatment.

2014-04-17 | E-GEOD-56864 | biostudies-arrayexpress

Proteomic analysis of plasmodesmata from Populus cell suspension cultures

Project description:In this project, we have investigated the proteome of the plasmodesmata (PD) enriched membrane fraction from poplar cell suspension cultures and quantitatively compared its protein composition with other membrane fractions. We build on the previously published literature on the PD proteome by using semiquantitative methods and, for the first time, demonstrate in vitro callose synthase activity from Populus PD proteins. To our knowledge, this study is the first proteomic investigation of the PD performed on a tree species.

2018-12-31 | PXD010692 | Pride

Grapevine cell cultures transcriptomic response to methyl jasmonate and cyclodextrin elicitors

Project description:The specificity and synergisms of methyl jasmonate and cyclodextrin elicitor effects in grapevine were analysed at the transcrptomic level. To this end, 12-14 day old cell cultures established from Vitis vinifera L. cv Monastrell calli were elicited with 50 mM cyclodextrin (CD), 100 µM methyl jasmonate (MJ) or the combination of both elicitros (MJ+CD). The experiment was carried out in triplicate and untreated cell cultures were followed in parallel as a control. Cells of each culture were isolated just prior and 24h after the treatment to obtain RNA. GrapeGen GeneChip microarray hybridization was performed from each sample. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Pablo Carbonell-Bejerano. The equivalent experiment is VV44 at PLEXdb.]

2015-11-15 | GSE42312 | GEO

Hybrid_Poplar_H11-11_Leaves_Local_Stress_Response_DataSet2

Project description:We investigated the transcriptional response of hybrid poplar (Populus trichocarpa x deltoides) leaves to methyl jasmonate treatment over a 24 hour time course. Experiments were conducted using clonal trees under greenhouse conditions at the University of British Columbia. We used the 15.5K poplar cDNA microarray platform previously described by Ralph et al. (Molecular Ecology 2006, 15:1275-1297). Differentially expressed genes were determined using three criteria: fold-change between methyl jasmonate-treated and tween-treated control leaves > 1.5-fold, P value < 0.05 and Q value < 0.05. This study identified > 1,000 differentially expressed genes in response to methyl jasmonate treatment.

2008-12-31 | GSE14039 | GEO